Ruiz-Monroy NM, López-Roblero A, Nájera-Ocampo M, Camacho-Vázquez C, Arce-Sillas A, Casanova-Hernández D, Palafox-Fonseca H, Melo-Salas M, Fragoso G, Sciutto-Conde E, Adalid-Peralta L

Idioma: Español
Referencias bibliográficas: 32
Paginas: 33-37
Archivo PDF: 192.65 Kb.

RESUMEN

Objetivo: determinar el papel de las células T reguladoras (Treg) durante la infección del cisticerco de taenia crassiceps en ratones C57BL/6. Material y métodos: se emplearon ratones hembras de 4 a 5 semanas de edad, de la cepa C57BL/6J. Se formaron dos grupos de ratones; se inoculó a cada ratón del grupo I por vía intraperitoneal con 20 cisticercos; el grupo II, sin infectar, se empleó como control. A diferentes tiempos de infección (5, 30, 90 y 130 días) se determinó el nivel de células Treg en el peritoneo, ganglios mesentéricos, placas de Peyer y el bazo. Paralelamente se evaluó la capacidad proliferativa de las células del bazo, estimulándolas con concanavalina A (ConA) y antígenos de T. crassiceps. Resultados: se observó que los niveles de Tregs fueron similares entre el grupo control y el infectado en el sitio de instalación del parásito (peritoneo) durante el periodo de estudio. Al mismo tiempo, se observaron niveles similares de proliferación en ambos grupos cuando las células se estimularon con ConA. Los antígenos específicos de cisticerco no indujeron proliferación de células T efectoras ni de Tregs. Estos resultados demuestran que el parásito T. crassiceps promueve una disminución en la capacidad proliferativa de linfocitos T, que es independiente de células Treg antígenoespecíficas. Conclusiones: la resistencia a la infección por el cisticerco de T. crassiceps en ratones de la cepa C57BL/6 no está asociada a presencia de células Treg.

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