2013, Number s1
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Arch Neurocien 2013; 18 (s1)
A prospective proteomic-based study for identifying potential biomarkers for the diagnosis of damage in focal cerebral ischemia induced in a rat model
Sánchez-Hernández H, Vite-Bazan CO, Heras-Romero Y, Cázares Raga FE, Cortés Martínez l, Ortiz PA, Hernández-Hernández FC
Language: Spanish
References: 22
Page: 27-32
PDF size: 965.61 Kb.
ABSTRACT
Cerebral vascular diseases are the third cause of death
in Mexico and around the world; including focal cerebral
ischemia (FCI) that leads to reduced blood supply in a
specific zone of the brain, diminishing the glucose and
oxygen. In early FCI stages there are molecular changes not well described in the cells of the affected and
surrounding regions; these changes could be detected
and used as biomarkers in easily accessed corporal fluids
as blood serum (BS), in order to define biomarkers useful
for disease advance diagnostic.
Objective: analyze protein
profile of rat sanguineous serum after 1h of FCI with or
without 24h of sanguineous reperfusion (rpf), in order to
identify possible early FCI biomarkers.
Material and
methods: cerebral focal ischemia was experimentally
induced in brain rat, BS was obtained and serum albumin
and immunoglobulin were depleted. Protein profile of
serum samples were obtained by two dimensional gel
electrophoresis, the images were analyzed using
ImageMaster 2D Platinum software allowing to identify
proteins present differentially in FCI.
Results: under FCI
and rpf, we observed up regulation of five proteins and
down regulation of three proteins. To analyze their role
as biomarkers in FCI, those proteins will be identified by
mass spectrometry.
Discussion and conclusion: protein
analysis of corporal liquids is useful to find biomarkers
to diagnostic and disease control. Proteins changes
expression and localization with FCI aggrieving or
dismissing injury, and BS could contain diagnostic
markers to FCI useful to identify early changes in neurons
and glial cells.
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