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2013, Number 4

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Rev Hematol Mex 2013; 14 (4)

Molecular characterization of FLT3-ITD mutations in Colombian patients with acute myelogenous leukemia

Jiménez-Mejía AM, Muskus C, Torres JD, Cuéllar-Ambrossi F, Camargo-Guerrero M, Vásquez-Palacio G

Language: Spanish
References: 22
Page: 166-172
PDF size: 454.07 Kb.


ABSTRACT

Background: In the past years, several studies detected high frequencies (15-30%) of mutations in the FLT3 gene in AML cases, which has prognosis implications. In Colombia, the frequency and type of mutations in FLT3 in AML cases is still unknown.
Objective: The aim of this study was to determine the frequency and type of mutations in the FLT3 gene that might be involved in the leukomogenesis in a cohort of Colombian AML patients.
Methodology: Descriptive and cohort study made in Colombia genomic DNA was isolated from mononucleated cells of AML patients peripheral blood or bone marrow using QIAamp DNA Blood Mini Kit. Mutations in juxtamembrane and tyrosine kinase domains of FLT3 gene were sequenced.
Results: Thirty-one AML patients were included in the present study. We found 3/31 with FLT3 ITD mutation. The patients with the mutation FLT3 ITD showed higher white blood counts.
Conclusions: This is the first study in Colombia that has been undertaken to determine mutations in FLT3 gene in our population. The frequencies of mutations in FLT3 ITD gene were lower (9.7%) than those informed in the literature. Besides, the presence of FLT3 ITD was associated with higher leukocyte counts in peripheral blood (p=0.023). The results of the present study entail the necessity of multicenter studies in our country to determine the clinical significance of these mutations in the Colombian population.


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