2013, Number 3
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Rev Cubana Farm 2013; 47 (3)
Genotoxic assessment of the Carapa guianensis Aublet seed oil extract in the chromosomal aberrations assay performed in Balb/c mice
Arencibia ADF, Rosario FLA, Delgado RL, Alonso LA, Infante BJF, Vidal NA
Language: Spanish
References: 33
Page: 363-367
PDF size: 100.86 Kb.
ABSTRACT
Introduction: the oil extract from
Carapa guianensis seed has various biomedical applications. It was recently evaluated and revealed great potentialities as antioxidant in
in vivo assays, but little is known about its effect on DNA in experimental biomodels.
Objective: to evaluate the genotoxic potential of the oil extract from Carapa guianensis seed in the chromosomal aberration of the bone marrow cells test performed in Balb/c mice.
Methods: five experimental groups were created: one placebo group (Tween 65, 2 %), three treated with different extract doses (400, 1000 and 2000 mg/kg) orally administered for 14 days and one positive control group treated with cyclophosphamide at a dose of 50 mg/kg intraperitoneally at 48 and 24 h before euthanasia. Five animals per sex from each group were administered the set dose. After 14 days of treatment, the animals were euthanized through cervical dislocation and their femoral bone marrow was taken out to perform the cytogenetic chromosomal aberration technique.
Results: the results between the control group and the groups treated with the extract did not differ between the two sexes in terms of the mitotic index variables, Gaps, polyploidy cells, number of cells with chromosome and chromatic aberrations and the percentage of aberration cells. However, the results of controls and of treated groups were different from those of the group treated with cyclophosphamide, which proved the validation of our results.
Conclusions: the oil extract from Carapa guianensis seeds does not have genotoxic potential for the formation of chromosome aberrations, mainly structural, in Balb/c mice bone marrow of both sexes.
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