Delgado DI, Barrera VM, Rodríguez BN, Sánchez ML, Mendoza ES, Ciprián CA

Language: English/Spanish
References: 29
Page: 295-302
PDF size: 495.79 Kb.

ABSTRACT

Early diagnosis is one of the main strategies in programs against different diseases that affect health and production of livestock. Bovine Herpes Virus 1 (BHV-1) is an important cause of morbidity and mortality in cattle and the Bovine Parainfluenza 3 (PI-3) virus causes a febrile, highly contagious pneumonia of acute course affecting different types of cattle. Direct immunoperoxidase (DIP) and direct immunofluorescence (DIF) tests for the diagnosis of BHV-1 and PI-3 in nasal secretions were standardized and established at CENSA. The diagnostic kits were validated, inspected and registered. The kits were useful to detect viral contamination in cell cultures; as well as for the identification of calves free of PI-3 and HPV-1, for diagnostic services in preputial washes of seroreactive bulls during artificial insemination; and for the differential diagnosis of vesicular diseases. Samples were taken from animals with respiratory signs to determine the role of these agents in the etiology of pneumonia cases from Havana and Granma provinces. It was found that the PI-3 virus had greater involvement. The kits proved to be useful for their rapidity, sensitivity and specificity. They are less time consuming and less expensive than viral isolation. It is recommended to extend the use of these kits in order to determine the actual role of BHV-1 and PI-3 viruses in different diseases found in this species, since screening for these viruses would be difficult using viral isolation.

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