medigraphic.com
ISSN 1870-199X (Print)
Órgano oficial de la Facultad de Odontología, UNAM

2013, Number 4

<< Back Next >>

Rev Odont Mex 2013; 17 (4)

Effect of lipoteichoic acid on gene expression in mice (H9c2) cardiomyocite

Adam BI, Gutiérrez-Venegas G, Ballesteros VA

Language: Spanish
References: 25
Page: 228-234
PDF size: 264.18 Kb.


ABSTRACT

Most dental pulp diseases and diseases of tissues surrounding the root are somehow related to micro-organisms. Peptidoglycans and lipoteichoic acid are two of the main Gram-positive bacteria components with activities related to sepsis development. When tissues sustain microbial invasion the host responds with both unspecific inflammatory defenses and specific immunological reactions. Surgical and non surgical endodontic treatments are essentially debridement procedures intended to destroy and eliminate the microbial eco-system associated to the pathological process. It is essential for clinicians to understand the intimate relationship existing between micro-organisms and endodontic disease, so as to be able to tailor a rational and effective treatment especially in subjects susceptible to infective endocarditis processes. In the present study research was conducted on TNFα, IL-1 COX-2 expression through the effect of lipoteichoic acid (LTA) of Streptococcus sanguinis by characterizing intra-cellular signals involved in H9c” cardiomyocytes. The cell line was treated with LTA at different concentrations during 30 minutes. When compared to control group, responses to LTA treatment were dependent on dosage. That expression was assessed by means of a One Step RT-PCR (Invitrogen) analysis. It was noted that the aforementioned expression resembled the organisms’s physiological response during an infective endocarditis episode and to exacerbation observed during an endodontic procedure.


REFERENCES

  1. Chávez de Paz L. Redefining the persistent infection in root canals: possible role of biofilm communities. JOE. 2007; 33 (6).

  2. Burns RCS. Vías de la pulpa. 7a. ed. Harcourt. 1999.

  3. Peña L. Inmunología. Manual Moderno. España. 1999.

  4. Venegas G, Cardoso P. Ácido lipoteicoico: receptores y mecanismo de transducción. REB. 2006; 25: 002.

  5. Seung HH, Je Hak K, Martin M, Michalek S, Nahm M. Pneumococcal lipoteichoic acid (LTA) is not as potent as staphylococcal LTA in stimulating toll like receptor 2. Infection and Inmunity. 2003; 71: 10.

  6. Guyton and Hall. Manual del tratado de fisiología médica. McGraw Hill Interamericana. México. 1999.

  7. Swantek JL, Tsen MF, Cobb JA. IL-1 receptor associated kinase modulates host responsiveness to endotoxin. J Inmunol. 2000; 164: 4301.

  8. Woodcock EA, Matkovich SJ. Cardiomyocytes structure, function and associated pathologies. Int J Biochem Cell Biol. 2005; 37.

  9. Beshay NM, Zordoky OS. H9c2 cell line is a valuable in vitro model to study the drug metabolizing enzymes in the heart. Journal of Pharmacological and Toxicological Methods. 2007; 56.

  10. Anderson R, Becker A. El corazón. Estructura normal y patológica. Mosby Doyma. México. 1994.

  11. Weine F. Tratamiento endodóncico. 5a. ed. Madrid. España. Harcourt. 2000.

  12. Hargreaves KM, Goodis HE. Seltzer and Bender’s dental pulp. Quintessence Books. 2002.

  13. Murray P, Smyth T et al. Analysis of pulpar reactions to restorative procedures, materials, pulp capping and future therapies. Crit Rev Oral Bio Med. 2002; 13 (6): 509-520.

  14. Ontiveros AG, Gutiérrez G, Lazo MR. Efecto del lipopolisacárido (LPS) y el ácido lipoteicoico (LTA) sobre la ciclooxigenasa-2 (COX-2) en células pulpares humanas. Revista Odontológica Mexicana. 2008; 12 (4): 177-183.

  15. Telles, P. Hanks, C. lipoteichoic acid up regulates VEGF expression in macrophages and pulp cells. J Dent Res. 2003; 82: 466-470.

  16. Siren E, Haapasalo M, Ranta K, Salmi P, Kerosuo E. Microbiological findings and clinical treatment procedures in endodontic cases selected for microbiological investigation. Int Endod J. 1997; 30 (2): 91-95.

  17. Siqueira J. Aetiology of root canal treatment failure: why well-treated teeth can fail. Int Endod J. 2001; 34: 1-10.

  18. Sjogren U, Figdor D, Persson S, Sundqvist G. Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis. Int Endod J. 1997; 30 (5): 297-306.

  19. Chavez De Paz L, Dahlen G, Molander A, Moller A, Bergenholtz G. Bacteria recovered from teeth with apical periodontitis after antimicrobial endodontic treatment. Int Endod J. 2003; 36 (7): 500-508.

  20. Peciuliene V, Balciuniene I, Eriksen HM, Haapasalo M. Isolation of Enterococcus faecalis in previously root-filled canals in a Lithuanian population. J Endod. 2000; 26 (10): 593-595.

  21. Peciuliene V, Reynaud A, Balciuniene I, Haapasalo M. Isolation of yeasts and enteric bacteria in root-filled teeth with chronic apical periodontitis. Int Endod J. 2001; 34 (6): 429-434.

  22. Siqueira JF, Rocas I, Souto R, de Uzeda M, Colombo A. Actinomyces species, streptococci, and Enterococcus faecalis in primary root canal infections. J Endod. 2002; 28 (3): 168-172.

  23. Rocas I, Siqueira J, Santos K. Association of Enterococcus faecalis with different forms of periradicular diseases. J Endod. 2004; 30: 315-20.

  24. Wilson, Walter et al. Prevention of infective endocarditis. Journal of American Heart Association. 2007; 116: 1736-1754.

  25. Meyer, D. Taylor, P. Oral Pathogens: from dental plaque to cardiac disease. Current Opinion in Microbiology. 1998; (1): 88-95.




2020     |     www.medigraphic.com