Cytotoxic impact of silver and silver diamine fluoride in six oral cells culture

René García-Contreras; Rogelio José Scougall-Vilchis; Rosalía Contreras-Bulnes; Hiroshi Sakagami; Julia Selene Baeza-Robleto; Rosa Isela Flores-Chávez; Hiroshi Nakajima

2013, Number 3

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Rev ADM 2013; 70 (3)

Cytotoxic impact of silver and silver diamine fluoride in six oral cells culture

García-Contreras R, Scougall-Vilchis RJ, Contreras-Bulnes R, Sakagami H, Baeza-Robleto JS, Flores-Chávez RI, Nakajima H

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Language: Spanish
References: 27
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ABSTRACT

The use of silver compounds in dentistry has been important in the development of dental materials science due to their potential antibacterial effect; however, the cytotoxic effect at a cellular and molecular level has not been adequately defined. Aim: To understand the cytotoxic impact of Ag(NH₃)₂F (Saforide) and AgCl in the culture of human pulp cells (HPC), human periodontal ligament cells (HPLC), human gingival fibroblasts (HGF), and squamous carcinoma cells obtained from three different patients (HSC-2, 3, and 4),using a quick MTT colorimetric bioassay. Material and methods: The cells were cultured in DMEM medium at different densities; Ag(NH₃)₂F (Saforide) and AgCl were inoculated at different concentrations and, after being in contact with the cells for 24 hours, cell viability was determined by means of a quick MTT colorimetric bioassay; the induced formazan was dissolved in dimethyl sulfoxide (DMSO) and tested in a microplate reader to estimate the number of living cells. The percentage, mean and standard deviation were calculated and statistical analysis was performed using the Kruskall-Wallis test. Results: After having been in contact with the Ag(NH₃)₂F for 24 hours, the results in terms of most sensitive cells were as follows: HSC-3›HPC›HSC-2›HSC-4›HGF›HPLF, the minimum dose needed to induce toxicity being 0.0097 mM. As regards sensitivity to pure silver (AgCl), the results were: HPC›HGF›HSC-3›HSC-2›HPLF›HSC-4, the mean dose required to induce toxicity being virtually the same in the various 0.25 mM cell lines. A slight hormesis effect was observed in the HGF. Conclusions: Silver compounds are widely used in dentistry; however, cytotoxicity may result in bioincompatibility and importance should be given to the risk/benefit factor during the clinical application of the compounds analyzed.

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