Carrillo-Esper R, Carrillo-Córdova CA, Carrillo-Córdova JR, Carrillo-Córdova LD

Language: English
References: 24
Page: 10-14
PDF size: 160.11 Kb.

ABSTRACT

Introduction. Advances in storage techniques of erythrocyte concentrates have contributed to develop significant erythrocyte morphologic abnormalities which have been associated with greater damage in the microcirculation, multiple organ dysfunction and increased morbidity and mortablity. Objective. To evaluate the changes in erythrocyte morphology related to storage time. Material and methods. We analyzed the morphologic changes in erythrocyte in relation to the storage time of the globular packages that were transfused. The blood smear was wisdom with Wright’s technique and was evaluated by two observers who evaluated the percentage of morphologic alterations. Results. 67 samples were collected. The average of abnormalities in the erythrocyte package was 40%. The storage time was of 12.25 ± 6.9 days. The Pearson correlation coefficient demonstrated correlation between the percentages of erythrocyte abnormalities and storage time. After seven days of storage 26.57% of the erythrocytes of the packages had morphological abnormalities which increased significantly in the following days. Conclusions. Red blood cells with more than seven days of storage, present significant morphologic alterations, erythrocyte abnormalities

REFERENCES

1. Wolfe LC. The membrana and the lesions of storage in preserved red cells. Transfusion 1985; 25: 185-203.

2. Tinmouth A, Chin-Yee I. The clinical consequences of the red cell storage lesion. Transfus Med Rev 2001; 15: 91-107.

3. Ho J, Sibbald WJ, Chin-Yee IH. Effects of storage on efficacy of red cell transfusion: when is it not safe? Crit Care Med 2003; 31: 687-97.

4. Loutit JF, Mollison PL, Young JM. Citric acid-sodium citrateglucose mixtures for blood storage. Q F Exp Physiol 1943; 32: 183-202.

5. Heaton A, Miripol J, Aster R. Use of Adsol preservation solution for prolonged storage of low viscosity AS-1 red blood cells. Br J Haematol 1984; 57: 467-78.

6. Simon TL, Marcus CS, Mythe BA. Effects of AS-3 nutrient-additive solution on 42 and 49 days storage of red cells. Transfusion 1987; 27: 178-82.

7. Kuduvalli M, Oo AY, Newall N. Effect of peri-operative red blood cell transfusion on 30 day and 1 year mortality following coronary artery bypass surgery. Eur J Cardiothorac Surg 2005; 27: 592-8.

8. Purdy FR, Tweeddale MG, Merrick PM. Association of mortality with age of blood transfused in septic ICU patients. Can J Anaesth 1997; 44: 1256-61.

9. Martin CM, Sibbald WJ, Lu X. Age of transfused blood is an independent risk factor for postinjury multiple organ failure. Am J Surg 1999; 178: 570-2.

10. Card RT. Red Cell membrane changes during storage. Trans Med Rev 1988; 2: 40-7.

11. Chin-Yee I, Arya N, d’Almeida MS. The red cell storage lesion and its implication for transfusion. Transfus Sci 1997; 18: 447-58.

12. Card RT, Mohandas N, Perkins HA. Deformability of stored red blood cells: relationship to degree of packing. Transfusion 1982; 22: 96-101.

13. Nakao M, Nakao T, Yamazoes S. Adenosine triphosphate and maintenance of shape of human red cells. Nature 1960; 187: 945-7.

14. Swietochowska K, Piascik R, Jaroszewicz K. Human stored blood inositol phospholipids. Acta Physiol 1991; 78: 283-91.

15. Dern RJ, Brewer GJ, Wiorkowski JJ. Studies of the preservation of human blood. The relationship of erythrocyte adenosine triphosphate levels. J Lab Clin Med 1967; 69: 968-78.

16. Greenwalt TJ, Bryan DJ, Dumaswala UJ. Erytrocyte membrane vesiculation and changes in membrane composition during storage in citrate-phosphate-dextrose-adenine. Vox Sang 1984; 47: 261-70.

17. Brunauer LS, Moxness MS, Huestis WH. Hydrogen peroxide oxidation induces the transfer of phospholipids from the membrane into the cytosol of human erythrocytes. Biochemistry 1994; 33: 4527-32.

18. Wagner GM, Chiu DT, Qju JH. Spectrin oxidation correlates with membrane vesiculation in stored RBC’s. Blood 1987; 69: 1777-81.

19. Heaton A, Keegan T, Holme S. In vivo regeneration of red blood cell 2,3-diphosphoglycerate following transfusion of DPG depleted AS-1, AS-3 and CPDA-1 red blood cells. Br J Haematol 1969; 71: 131-6.

20. Bunn HF, May MH, Kocholaty WF. Hemoglobin function in stored blood. J Clin Invest 1969; 48: 311-21.

21. Hebert PC, Wells G, Blajchman MA. A multicenter, randomized, controlled clinical trial of transfusion requirements in critical care. Transfusion Requirements in Critical Care Investigators. N Engl J Med 1999; 340: 409-17.

22. Vincent JL, Baron JF, Reinhart K. Anemia Blood transfusion critically ill patients. JAMA 2002; 288: 1499-507.

23. Corwin HL, Gettinger A, Pearl RG. The CRIT study: Anemia and Blood Transfusion in the Critically Ill. Current Practice in the United States. Crit Care Med 2004; 32: 39-52.

24. Practice Guidelines for the Blood Component Therapy. A Report by the American Society of Anesthesiologists Task Force on Blood Component Therapy. Anesthesiology 1996; 84: 732-47.