2002, Number 2
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Microbiología 2002; 44 (2)
Determination of the profile of fatty acids of 4 species of Shigella spp by chromatography of gases
Robles VE, Hurtado BMD, González AME, Ramírez GP, Durán DA, Ayala PR, Martínez PME
Language: English
References: 26
Page: 69-74
PDF size: 62.12 Kb.
ABSTRACT
Shigella boydii, Shigella flexneri, Shigella dysenteriae and
Shigella sonnei were identified using gas chromatography instead of the traditional techniques. Their acid methyl esters profiles were determined using a gas chromatograph Hewlett Packard 5890A and a RSL-150 heliflex capillary column. A total of 192 samples were analyzed both reference strains (ATCC 8700, INDRE B2188, B2194 and B2199) and environmental isolates. 12 fatty acids were included in the profiles from which 3-hydroxytetradecanoic acid (peak 12), trans 9-octadecanoic acid (peak 22), heptadecanoic acid (peak 18) and octadecanoic acid (peak 23), were the most important for the differentiation of the species analyzed.
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