Robles VE, Hurtado BMD, González AME, Ramírez GP, Durán DA, Ayala PR, Martínez PME

Idioma: Ingles.
Referencias bibliográficas: 26
Paginas: 69-74
Archivo PDF: 62.12 Kb.

RESUMEN

Con el objeto de identificar Shigella boydii, Shigella flexneri, Shigella dysenteriae y Shigella sonnei, utilizando la técnica de cromatografía de gases en sustitución de las pruebas tradicionales, se compararon los perfiles de ácidos grasos a través de sus ésteres metílicos; que se obtuvieron en un cromatógrafo de gases Hewlett Packard 5890A con columna capilar RSL-150 heliflex. Se realizaron un total de 192 análisis en muestras tanto de cepas de referencia (ATCC 8700, INDRE B2188, B2194 y B2199) como de cepas ambientales, determinándose que su perfil cromatográfico está constituido por 12 ácidos grasos, de los cuales el 3-hydroxitetradecanoico (pico 12), el trans 9-octadecanoico (pico 22), el heptadecanoico (pico 18) y el octadecanoico (pico 23) resultaron ser determinantes para la diferenciación entre las especies estudiadas.

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