2002, Number 1
<< Back Next >>
Microbiología 2002; 44 (1)
Virulence factors of A. caviae strains isolated from acute diarrheic disease in Cuba
Castro EG, Peña DD, Castañeda N, García AA, Morier DL, Aguilera-Arreola MG, Bravo FL
Language: English
References: 15
Page: 11-13
PDF size: 44.00 Kb.
ABSTRACT
Fifty Aeromonas caviae strains from intestinal infection in different Cuban provinces were identified by the Aerokey II method and virulence factors were investigated. The strains did not produce haemolysins but other exoenzymes such as proteases, lipases, and DNases; additionally, all isolates adhered to the HEp-2 cell line by the Carrello method and this did not correlate with other virulence factors presence which demonstrates that the haemolysin phenotypic expression is not necessary for these strains to be pathogenic and that pathogenicity is multifactorial, each strain expressing at least one virulence factor.
REFERENCES
Blazevic D.J. & G.M. Ederer. 1975. Principles of biochemical test in diagnostic microbiology. New York, J Wiley. pp: 13-14,110-111; 91-94, 127-128; 69-72, 122; 122-127.
Brender R. & J.M. Janda.1987. Detection quantification and stability of the b-haemolysin of Aeromonas spp. J. Med. Microbiol. 24: 247-251.
Carnahan, A.M., S. Behram & S.W. Joseph. 1991. Aerokey II: A Flexible Key for Identificating Clinical Aeromonas Species. J. Clin. Microbiol. 29(12):2843-2849.
Carrello A., K. Solburn, J. Budden & B. Chang. 1988. Adhesion of clinical and environmental Aeromonas isolates to Hep-2 cells. J. Med. Microbiol. 26:19-27.
Figura N. & P. Guglielmetti. 1987. Differentiation of motile and mesophilic strains into species by testing for a Camp like factor. J. Clin. Microbiol. 25:13411342.
Figura N., L. Marry, S. Verdiani, C. Ceccherini & A. Barberi. 1986. Prevalence, species differentiation and toxigenicity of Aeromonas strains in cases of childhood gastroenteritis and in controls. J. Clin. Microbiol. 23:595-99.
Karunakaran T. & B. Deri. 1995. Protolytic activity of Aeromonas caviae. J. Basic. Microbiol. 35:241-47.
Kirov, S.M., L.A. O’Donovan & K. Sanderson. 1999. Functional Characterization of Type IV Pili Expressed on Diarrhea-Associated Isolates of Aeromonas species. Infect Immun. 67(10):5447-5454.
Ljungh A. & T. Wadström. 1985. Aeromonas hydrophila an accepted enterotoxigenic “Weoenteropathogen”?. J. Infect. Dis. 151:972-73.
Millership, S.E., M.R. Barer & S. Tabaqchali. 1986. Toxin production by Aeromonas spp. from different sources. J. Med. Microbiol. 22(4):311-314.
Namdari H. & E. Bottone. 1990. Microbiol and Clinical Evidence Supporting the role of Aeromonas caviae as a pedriatric enteric pathogen. J. Clin. Microbiol. 28:837-840.
Nishikawa Y., T. Kimura & T. Kishi. 1991. Mannose resistant adhesion of motile Aeromonas to INT 407 cells and differences among isolates from humans, food and water. Epidemiol Infect. 107:171-179.
Santos, A.J., C.J. González, A. Otero & M.L. García-López. 1999. Hemolytic Activity and Siderophore Production in Different Aeromonas Species Isolated from Fish. Appl. Environ. Microbiol. 65(12):5612-5614.
Watson, I.M., J.O. Robinson, V. Burke & M. Gracey. 1985. Invasiveness of Aeromonas spp. in Relation to Biotype, Virulence Factors, and Clinical Features. J. Clin. Microbiol. 22(1):48-51.
Wang, G., K.D. Tyler, C.K. Munro & W.M. Johnson. 1996. Characterization of Cytotoxic, Hemolytic Aeromonas caviae Clinical Isolates and Their Identification by Determining Presence of a Unique Hemolysin Gene. J. Clin. Microbiol. 34(12):3203-3205.