medigraphic.com
Revista Latinoamericana de Microbiología

2002, Number 3-4

<< Back Next >>

Microbiología 2002; 44 (3-4)

Comparison between a commercial and an in-house ELISA for anti-M. avium paratuberculosis antibodies detection in dairy herds in Rio de Janeiro, Brazil

Ferreira R, Fonseca L, Lilenbaum W

Language: English
References: 24
Page: 129-132
PDF size: 54.09 Kb.


ABSTRACT

Paratuberculosis (Johne’s disease) is a chronic enteritis that affects ruminants and is caused by Mycobacterium avium subsp. paratuberculosis (Map). The disease is worldwide spread and causes important economic losses. In Brazil, Map has already been isolated, but there are no enough epidemiological studies about its prevalence. In this study, 179 sera of dairy cows originated from Rio de Janeiro State, Brazil, were tested for the presence of anti- Map antibodies using two ELISAs. First one was an “in house” ELISA using a protoplasmic paratuberculosis antigen (PPA – Allied Monitor) as capture antigen and a monoclonal anti-bovine IgG conjugated to alkaline phosphatase (Sigma) and the other was “HerdChek Mpt” (IDEXX), used as standard method in this study. Fifty-nine (32.9%) samples were reactive in the PPA-ELISA. Sensitivity of ELISA-PPA in relation to HerdCheck was 47.5% and specificity was 86.8%, with 54/103 of false negatives and 10/76 of false positives. Positive predictive value of PPA-ELISA was calculated as 86% and efficiency as 64.2%. PPA-ELISA and HerdChek results were also compared by kappa test (k) and concordance index was 0.320. PPA-ELISA can be useful as a collective herd test and can be recommended as a screening test for identification of infected herds and foci of Johne’s disease.


REFERENCES

  1. Boelaert, F.; Walravens, K.; Biront, P.; Vermeersch, J.P.; Berkvens, D. and Godfroid, J. 2000. Prevalence of paratuberculosis (Johne’s disease) in the Belgian cattle population. Vet. Microbiol. 77:269-281.

  2. Cocito, C.; Gilot, P.; Coene, M.; De Kesel, M.; Poupart, P and Vennuffel. P. 1994. Paratuberculosis. Clin. Microbiol. Rev. 7(3):328-345.

  3. Collins, M.T.; Sockett, D.C.; Ridge, S. and Cox, J.C. 1991. Evaluation of a commercial enzime-linked immunosorbent assay for Johne’s disease. J. Clin. Microbiol. 29(2):272-276.

  4. Collins, M.T. and Sockett, D.C. 1993. Accuracy and economics of the USDA-licensed enzyme-linked immunosorbent assay for bovine paratuberculosis. J. Am. Vet. Med. Ass. 203 (10):1456-1463.

  5. Dolz, G.; Araya, L.N.; Suárez, J. and Jiménez. C. 1999. Prevalence of antibodies to bovine paratuberculosis detected by a LAM-ELISA in Costa Rica. Vet. Rec. 144:322-323.

  6. Eamens, G.J.; Whittington, R.J.; Marsh, I.B.; Turner, M.J.; Saunders, V.; Kemsley, P.D. and Rayward, D. 2000. Compararive sensitivity of various faecal culture methods and ELISA in dairy herds with endemic Johne’s disease. Vet. Microbiol. 77:357-367.

  7. Ferreira, R.; Fonseca, L.S. and Lilenbaum, W. 2001. Detecção de anticorpos contra Mycobacterium paratuberculosis em rebanhos bovinos do Estado do Rio de Janeiro, Brasil. Rev. Bras. Med. Vet. 23:166-171.

  8. Ferreira, R.; Fonseca, L.S. and Lilenbaum, W. 2002. Agar Gel Immunodiffusion test (AGID) evaluation for detection of bovine paratuberculosis in Rio de Janeiro, Brazil. Lett. Appl. Microbiol. 35: 163-175.

  9. Fonseca, L.F.L.; Santos, M.V.; Pereira, C.C.; Olival, A.A.; Hienemann, M.B. and Richtzenhain, L.J. 1999. Identificação da presença de anticorpos contra Mycobacterium paratuberculosis em rebanhos leiteiros do Estado de São Paulo. Arq. Inst. Biol. São Paulo, 66:122.

  10. Galen, R.S. and Gambino, S.R., 1975. Beyond Normality: The Predictive Value and Efficiency of Medical Diagnosis, (John Wiley and Sons, New York).

  11. Gasteiner, J.; Awad-Masalmeh, M. and Baumgartner, W. 2000. Mycobacterium avium subsp. paratuberculosis infection in cattle in Austria, diagnosis with culture, PCR and ELISA. Vet. Microbiol. 77:339-349.

  12. Goldberger, M. 1997. Regulatory issues for new devices. WHO Tuberculosis Diagnostic Workshop, Product Developement Guidelines. WHO. Cleveland, Ohio.

  13. Harris, N.B. and Barletts, R.G. 2001. Mycobacterium avium subsp. paratuberculosis in Veterinary Medicine. Clin Microbiol. Rev. 14 (3):489-512.

  14. Jubb, T.F. and Galvin, J.W. 2002. Testing to control Johne’s disease in dairy herds in Victoria. 7th. Int. Coll. Paratuberculosis. Bilbao, 11-14th June 2002. p. 126.

  15. Koets, A.P.; Retten, V.P.M.G.; De Boer, M.; Bakker, D.; Valentin-Weigand, P. and Van Eden, W. 2001. Differential Changes in Heat Shock Protein-, Lipoarabinomannan-, and Purified Protein Derivative-Specific Immunoglobulin G1 and G2 Isotype Responses during Bovine Mycobacterium avium subsp. paratuberculosis Infection. Infect. Immun. 69 (3):1492-1498.

  16. Muskens, J.; Barkema, H.W.; Russchen, E.; Van Maanen, K.; Schukken, Y.H. and Bakker, D. 2000. Prevalence and regional distribution of paratuberculosis in dairy herds in the Netherlands. Vet. Microbiol. 77:253-261.

  17. NAHMS, 1997. Johne’s Disease on U.S. Dairy Operations. USDA: APHIS: VS. CEAH, National Animal Health Monitoring System. Fort Collins, CO. #N245.1097.18. Ocepek, M.; Krt, B. and Pogacnik, M.2002. Seroprevalence of paratuberculosis in bull mothers in Eslovenia. 7th. Int. Coll. Paratuberculosis. Bilbao, 11-14th June 2002. p. 113.

  18. Sockett, D.C.; Conrad, T.A.; Thomas, C.B. and Collins, M.T. 1992. Evaluation of four serological tests for bovine paratuberculosis. J. Clin. Microbiol. 30:1134-1139.

  19. Stabel, J.R. (2000). Transitions in immune response to Mycobacterium paratuberculosis. Vet. Microbiol. 77:465-473.

  20. Stabel, J.R. 1998. Johne’s disease: a hidden threat. J. Dairy Sci. 81:283-288.

  21. Sugden, E.A.; Stilwell, K. and Michaelides, A. 1997. A Comparison of lipoarabinomannan with other antigens used in absorbed enzyme immunoassays for the serological detection of cattle infected with Mycobacterium paratuberculosis. J. Vet. Diagn. Investig. 9:413-417.

  22. Van Maanen, C.; Koster, C.; van Veen, B.; Kalis, C.H.J. and Collins, M.T. 2002. Validation of Mycobacterium paratuberculosis antibody detecting ELISAs. 7th. Int. Coll. Paratuberculosis. Bilbao, 11-14th June 2002. p. 61.

  23. Walravens, K.; Marché, S.; Malbrecq, S.; Rosseels, V.; Huygen, K. and Godfroid, J. 2002. Evaluation of a (lipo)polysaccharides absorbed ELISA for the serological diagnostic of M. paratuberculosis. 7th. Int. Coll. Paratuberculosis. Bilbao, 11-14th June 2002. p. 65.

  24. Whitlock, R.H.; Wells, S.J.; Sweeney, R. Wand and Van Tiem, J. 2000. ELISA and fecal culture for paratuberculosis (Johne’s disease): sensitivity and specificity of each method. Vet. Microbiol. 77:387-398.




2020     |     www.medigraphic.com