medigraphic.com
Revista Latinoamericana de Microbiología

2002, Number 3-4

<< Back Next >>

Microbiología 2002; 44 (3-4)

Evaluation of a rapid slide agglutination test for the diagnosis of acute canine leptospirosis

Lilenbaum W, Ristow P, Almeida FS, Domingues DSE

Language: English
References: 23
Page: 124-128
PDF size: 56.60 Kb.


ABSTRACT

The standard method for the diagnostic of leptospirosis is the microscopic agglutination test (MAT). Nevertheless, it is time-consuming and presents risk of infection for laboratory personal. Several attempts to simplify and reduce the time, skill and expense required for serological testing were made. Rapid slide agglutination tests (RSAT) have been used nowadays as a reliable screening test for the detection of acute and recent infections by many laboratories. The purpose of that study was to evaluate a new antigen preparation for the rapid diagnoses of acute canine leptospirosis and to compare it with MAT results in clinically suspect serum samples. Two hundred and thirteen serum samples from dogs of both sexes and different ages were tested and 141 (66.2%) animals were considered as positive by MAT. The most frequent serovar was icterohaemorrhagiae, followed by canicola. RSAT results were very similar to those observed on MAT, with 139 (65.3%) reactive animals. Correlation between RSAT and MAT was positive (0.82) and significant (p&#~8249;0,01). Concordance of results was of 93.4% (199/213) of the animals correctly diagnosed by RSAT. Considering MAT as the standard test, sensitivity of RSAT was calculated on 94.3% (133/141) and specificity on 91.7% (66/72).


REFERENCES

  1. Arimitsu, Y., Kmety, E., Ananyina, Y., Baranton, G., Ferguson IR, Smythe, I. and Terpstra, W.J. 1994. Evaluation of the one-point microcapsule agglutination test for diagnosis of leptospirosis. Bull. WHO, 72;395-399.

  2. Bolin, C.A. 1996. Diagnosis of Leptospirosis: A reemerging disease of companion animals. Sem. Vet. Med. Surg. 11:166-171.

  3. Brandao, A.P., Camargo, E.D., Silva, E.D., Silva, M.V. and Abrao, R.V. 1998. Macroscopic agglutination test for rapid diagnosis of human leptospirosis. J. Clin. Microbiol. 36:3138-3142.

  4. Cai HY, Hornby G, Key DW, Osuch MR, Maxie MG. 2002. Preliminary study on differentiation of Leptospira grippotyphosa and Leptospira sejroe from other common pathogenic leptospiral serovars in canine urine by polymerase chain reaction assay. Vet. Diagn. Invest. 14:164-168

  5. Caldas, E.M. and Sampaio, M.B. 1979. Leptospirosis in the city of Salvador, Bahia, Brazil. Int. J. Zoon. 6:85-96.

  6. Cordeiro, F. and Sulzer, C.R. 1983. Leptospira interrogans, serovar copenhageni, isolated from a dog in Belo Horizonte, Brazil. Rev Microbiol. 14:38-41.

  7. Faine, S., Adler, B., Bolin, C., Perolat, P. 2000. Leptospira and Leptospirosis. MedSci, Melbourne, Austrália, 2nd Ed.

  8. Galen R.S. and Gambino, C.R. 1975. Beyound normality: the predictive value and efficiency of medical diagnosis. John Wiley and Sons, New York.

  9. Galton, M.M., Powers, D.K., Hall, A.D. and Cornell, R.G. 1958. A rapid macroscopic-slide screening test for the serodiagnosis of leptospirosis. Am. J. Vet. Res. 72:505-512.

  10. Harkin, K.R. and Gartrell, C.L. 1996. Canine leptospirosis in New Jersey and Michigan: 17 cases (1990-1995). J. Am. Anim. Hosp. Assoc. 32: 495-501.

  11. Heat, S.E. and Jhonson, R. 1994. Leptospirosis. JAVMA, 205:1518-1523.

  12. Levett, P. Leptospirosis. 2001. Clin. Microbiol. Rev. 14;296-326.

  13. Lilenbaum, W., Ribeiro, V., Martin, E. and Bispo, V. 1993. Serological study for the detection of antibodies anti-leptospira in Rattus norvegicus from Duque de Caxias, Rio de Janeiro, Brazil. Rev. Latinoam. Microbiol. 35:357- 360.

  14. Lilenbaum, W., Ribeiro, V.L.S. and Brunstein, R. 1994. Leptospirosis in dogs clinically suspect in Rio de Janeiro, Brazil. Hora Veterinária, 81:48-49.

  15. Mazzonelli, J.D., Mazzonelli, M. and Mailloux, M. 1974. Possibilité de diagnostic sérologique macroscopique des leptospiroses à l’aide d’un antigéne unique. Med. Mal. Infect. 4:253-254.

  16. Nicolescu, M., Andreescu, N., Lupan, E. and Pop, A. 1982. The serological diagnosis of leptospirosis in domestic animals by the macroscopic agglutination test with patoc antigen. Arch. Roum. Path. Exp. Microbiol. 41:11-13.

  17. Nielsen, J.N., Cochran, G.K., Cassells, J.A., Phil, B. and Hanson, L.E. 1991. Leptospira interrogans serovar bratislava infection in two dogs. JAVMA. 199:351-352.

  18. Ramadass P, Samuel B and Nachimuthu K. 1999. A rapid agglutination test for detection of leptospiral antibodies. Vet Microbiol. 70:37-140

  19. Ribotta, M.J., Higgins, R., Gottschalk, M. and Lallier, R. 2000. Development of an indirect enzyme-linked immunosorbent assay for the detection of leptospiral antibodies in dogs. Can. J. Vet. Res. 64:32-37

  20. Sampaio, I.B.M. 1998. Statistics applied to animal experiments. First Edition. UFMG, Belo Horizonte, Brazil. Pp 221.

  21. Stoenner, H.G. and Davis, E. 1967. Further observations on leptospiral plate antigens. Am. J. Vet. Res. 28:259-266.

  22. Wolff, J.W. and Bohlander, J. 1967. Screening tests in human serum samples with Leptospira biflexa antigens incorporated in Galton’s macroscopic slide test. Trop. Geogr. Med. 19:63-69.

  23. Yanaguita, R.M. 1987. Diagnóstico rápido da leptospirose animal pela soroaglutinação macroscópica com antígenos de Leptospira biflexa. (Thesis). Universidade de São Paulo, Pp 68.




2020     |     www.medigraphic.com