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Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán

2012, Number 6

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Rev Invest Clin 2012; 64 (6)

qacEΔ1 gene frequency and biocide resistance in extended-spectrum β-lactamase producing Enterobacteriaceae clinical isolates

Pastrana-Carrasco J, Garza-Ramos JU, Barrios H, Morfin-Otero R, Rodríguez-Noriega E, Barajas JM, Suárez S, Díaz R, Miranda G, Solórzano F, Contreras J, Silva-Sánchez J

Language: Spanish
References: 34
Page: 535-540
PDF size: 177.68 Kb.


ABSTRACT

Objective. To determine the frequency of the gene qacEΔ1 and characterize the resistance to biocides of extendedspectrum β-lactamases producing enterobacteriaceae (ESBLPE) obtained from clinical isolates causing nosocomial infections. Material and methods. In total 59 ESBL-PE causing nosocomial infections were included: Klebsiella pneumoniae (35) and Enterobacter cloacae (24). Minimal inhibitory concentration (MIC) was tested for chlorhexidine (CHX) and benzalkonium chloride (CLBZ) by agar dilution technique. Amplification of the SHV, TLA-1 and qacEΔ1 genes were performed by PCR using specific primers and plasmid identification was done by alkaline lysis method. Matting experiments were obtained on solid agar method. Results. Chlorhexidine-resistance was found in 100% of the ESBL-PE and benzalkonium chloride-resistance in 80%. In 68% of the biocides-resistant strains the qacEΔ1 gene was present. The 66% of resulting transconjugants were resistant to CHX and the gene qacEΔ1 was detected in 55%. Conclusions. The qacEΔ1 gene of antiseptic resistance is widespread in the EP-ESBL and can be transferred horizontally. Thus it is advisable to use combinations of antiseptics, as recommended in the literature, to avoid selection of multiresistant bacteria in hospitals, causing nosocomial infections.


REFERENCES

  1. Russell AD. Biocide use and antibiotic resistance: the relevance of laboratory findings to clinical and environmental situations. The Lancet Infect Dis 2003; 3: 794-803.

  2. Hegstad K, Langsrud S, Lunestad BT, Scheie AA, Sunde M, Yazdankhah SP. Does the wide use of quaternary ammonium compounds enhance the selection and spread of antimicrobial resistance and thus threaten our health? Microb Drug Resist 2010; 16(2): 91-104.

  3. Ishikawa S, Matsumura Y, Yoshizako F, Tsuchido T. Characterization of a cationic surfactant-resistant mutant isolated spontaneously from Escherichia coli. J Appl Microbiol 2002; 92: 261-8.

  4. Hugo WB. In: Russell AD, Hugo WB, Ayliffe GA (eds.). Principle and practice of disinfection, preservation, and sterilization. Disinfectant mechanism. 2nd. Ed. Oxford, England: Blackwell Scientific Publications; 1992, p. 187-210.

  5. McDonnell G, Russell AD. Antiseptics and disinfectants: activity, action, and resistance. Clin Microbiol Rev 1999; 12: 147-79.

  6. Kazama H, Hamashima H, Sasatsu M, Arai T. Distribution of the antiseptic-resistance genes qacE and qacE delta 1 in Gramnegative bacteria. FEMS Microbiol Lett 1998; 159: 173-78.

  7. Wang C, Zhan Q, Mi Z, Huang Z, Chen G. Distribution of the antiseptic-resistance gene qacEDelta1 in 283 clinical isolates of Gram-negative bacteria in China. J Hosp Infect 2008; 69(4): 394-6.

  8. Paulsen IT, Littlejohn TG, Radstrôm P, Sundstrôm L, Skôld O, Swedberg G, et al. The 3’ conserved segment of integrons contains a gene associated with multidrug resistance to antisep tics and disinfectants. Antimicrob Agents Chemother 1993; 37: 761-8.

  9. Fang CT, Chen HC, Chuang YP, Chang SC. Wang JT. Cloning of a cation efflux pump gene associated with chlorhexidine resistance in Klebsiella pneumoniae. Antimicrob Agents Chemother 2002; 46: 2024-8.

  10. Livermore DM. β-lactamases in laboratory and clinical resistance. Clin Microbiol Rev 1995; 8: 557-84.

  11. Alcantar-Curiel D, Tinoco JC, Gayosso C, Carlos A, Daza C, Perez-Prado MC, et al. Nosocomial Bacteremia and Urinary Tract Infections Caused by Extended-Spectrum β-Lactamase Producing Klebsiella pneumoniae with Plasmids Carrying Both SHV-5 and TLA-1 Genes. Clin Infect Dis 2004; 38: 1067-74.

  12. Miranda G, Castro N, Leaños B, Valenzuela A, Garza-Ramos U, Rojas T, et al. Clonal and horizontal dissemination of Klebsiella pneumoniae expressing SHV-5 extended-spectrum β-lactamase in a Mexican pediatric hospital. J Clin Microbiol 2004; 42: 30-5.

  13. Preston KE, Venezia RA, Stellrecht KA. The SHV-5 extendedspectrum β-lactamase gene of pACM1 is located on the remnant of a compound transposon. Plasmid 2004; 51: 48-53.

  14. Silva J, Aguilar C, Becerra Z, López-Antunano F, García R. Extended spectrum beta-lactamases in clinical isolates of enterobacteria in Mexico. Microb Drug Resist 1999; 5(3): 189-93.

  15. Silva J, Gatica R, Aguilar C, Becerra Z, Garza-Ramos U, Velásquez M, et al. Outbreak of infection with Extended-spectrum β-lactamase producing Klebsiella pneumoniae in a Mexican hospital. J Clin Microbiol 2001; 39: 3193-6.

  16. Miranda G, Castro N, Leaños B, Valenzuela A, Garza-Ramos U, Rojas T, et al. Clonal and Horizontal Dissemination of Klebsiella pneumoniae Expressing SHV-5 Extended-Spectrum β- Lactamase in a Mexican Pediatric Hospital. J Clin Microbiol 2004; 42: 30-5.

  17. National Committee for Clinical Laboratory Standards. Methods for dilution antimicrobial susceptibility test for bacteria that grow aerobically. Approved standard M2-A6. Clinical Laboratory Standards Institute, Wayne, Pa. 2004.

  18. Jesús Silva-Sánchez, Jesús Ulises Garza-Ramos, Fernando Reyna- Flores, Alejandro Sánchez-Pérez, Teresa Rojas-Moreno, Verónica Andrade-Almaraz, et al. Extended-spectrum β-lactamase (ESBLs) producing Enterobacteriaceae causing nosocomial infections in Mexico, a retrospective and multicenter study. Arc Med Res 2011; 42(2): 156-62.

  19. Garza-Ramos U, Martínez-Romero E, Silva-Sánchez J. SHVtype extended-spectrum beta-lactamase (ESBL) are encoded in related plasmids from enterobacteria clinical isolates from Mexico. Sal Pub Mex 2007; 49(6): 415-21.

  20. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: A laboratory manual. 2nd. Ed. New York: Cold Spring Harbor Laboratory Press, Cold Spring Harbor; 1989.

  21. Garza-Ramos U, Martínez-Romero E, Silva-Sánchez J. SHVtype extended-spectrum beta-lactamase (ESBL) are encoded in related plasmids from enterobacteria clinical isolates from Mexico. Sal Pub Mex 2007; 49(6): 415-21.

  22. Kieser T. Factors affecting the isolation of CCC DNA from Streptomyces lividans and Escherichia coli. Plasmid 1984; 12: 19-36.

  23. Miller J (ed.). Nueva York: Experiments in molecular genetics. Cold spring Harbor Laboratory, Cold Spring Harbor; 1992, p. 82-5.

  24. Russell AD. Do biocides select for antibiotic resistance? J Pharm Pharmacol 2000; 52: 227-33.

  25. Boyce JM. New insights into improving hand hygiene practices. Infect Control Hosp Epidemiol 2004; 25: 187-8.

  26. Paulsen IT, Brown MH, Skurray RA. Proton-dependent multidrug efflux systems. Microbiol Rev 1996; 60: 575-608.

  27. Cattoir V, Nordmann P. Plasmid-mediated quinolone resistance in gram-negative bacterial species: an update. Curr Med Chem 2009; 16(8): 1028-46.

  28. Kazama H, Kizu K, Iwasaki M, Mamashima H, Sasatsu M, Arai T. Isolation and structure of a new integron that includes a streptomycin resistance gene from the R plasmid of Pseudomonas aeruginosa. FEMS Microbiol Lett 1995; 134: 137-41.

  29. Lambert RJ, Joynson J, Forbes B. The relationship and susceptibilities of some industrial, laboratory and clinical isolates of Pseudomonas aeruginosa to some antibiotics and biocides. J Appl Microbiol 2001; 91: 972-84.

  30. Tattawasart U, Maillard JY, Furr JR, Russell AD. Development of resistance to chlorhexidine diacetate and cetylpyridium chloride in Pseudomonas stutzeri and changes in antibiotic susceptibility. J Hosp Infect 1999; 42: 219-29.

  31. Berçot B, Poirel L, Silva-Sanchez J, Nordmann P. Association of the extended-spectrum beta-lactamase gene blaTLA-1 with a novel ISCR element, ISCR20. Antimicrob Agents Chemother 2010; 54: 4026-8.

  32. Coldham NG, Webber M, Woodward MJ, Piddock LJ. A 96- well plate fluorescence assay for assessment of cellular permeability and active efflux in Salmonella enterica serovar Typhimurium and Escherichia coli. J Antimicrob Chemother 2010; 65(8): 1655-63.

  33. Boyce JM. Antiseptic technology: access, affordability, and acceptance. Emerg Infect Dis 2001; 7: 231-33.

  34. Tarrand JJ, LaSala PR, Han XY, Rolston KV, Kontoyiannis DP. Dimethyl sulfoxide enhances effectiveness of skin antiseptics and reduces contamination rates of blood cultures. J Clin Microbiol 2012; 50: 1552-7.




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