medigraphic.com
ISSN 2007-4360 (Print)
Asociación Mexicana de Periodontología, Colegio de Periodoncistas A.C

2012, Number 2

<< Back Next >>

Rev Mex Periodontol 2012; 3 (2)

Effect of ciclosporin A on the expression and production of type I collagen in human gingival fibroblasts

Ríos VS, Cano S, Laclette JP, De la Torre P, Campos OA, Uribe-Querol E

Language: Spanish
References: 26
Page: 65-71
PDF size: 619.82 Kb.


ABSTRACT

Introduction: Cyclosporine A is an immunosuppressor used to prevent rejection of transplanted organs. About the 30% of the patients treated with this immunosuppressor develop gingival overgrowth. However, the precise cellular events that lead to the pathology are unknown. The aim of this study is to understand how cyclosporine A could be altering the expression and production of collagen I. Material and methods: Fibroblast cultures derived from a biopsy of a patient treated with cyclosporine A were used to document both the expression, by RT-PCR, and the production, by immunocytochemistry, of collagen I. Results: The amount of messenger RNA for collagen I was higher in gingival fibroblast of the gum of a patient treated with cyclosporine A than in gingival fibroblast of the control person. In concordance with this result, the amount of collagen expressed in gingival overgrowth fibroblast increased with respect to the control fibroblast. Conclusion: cyclosporine A induced the overexpression and overproduction of collagen type I by gum fibroblast.


REFERENCES

  1. Braun WE. Renal transplantation: Basic concepts and evolution of therapy. J Clin Apher 2003; 18: 141-152.

  2. Gonçalves SC, Diaz-Serrano KV, de Queiroz AM, Palioto DB, Faria G. Gingival overgrowth in a renal transplant recipient using cyclosporine A. J Dent Child (Chic) 2008; 75: 313-317.

  3. Hassell TM, Hefti AF. Drug-induced gingival overgrowth: old problem, new problem. Crit Rev Oral Biol Med 1991; 2: 103-137.

  4. Greenberg KV, Armitage GC, Shiboski CH. Gingival enlargement among renal transplant recipients in the era of new-generation immunosuppressants. J Periodontol 2008; 79: 453-460.

  5. Nakib N, Ashrafi SS. Drug-induced gingival overgrowth. Dis Mon 2011; 57: 225-230.

  6. American Academy of Periodontology. Glossary of periodontal terms. Chicago, Ill: American Academy of Periodontology; 1992.

  7. Newman MG, Takei HH, Carranza FnA. Carranza’s clinical periodontology. St. Louis, Mo: Elsevier/Saunders; 2012.

  8. Armitage GC. Development of a classification system for periodontal diseases and conditions. Ann Periodontol 1999; 4: 1-6.

  9. Livada R, Shiloah J. Gummy smile: Could it be genetic? Hereditary gingival fibromatosis. J Tenn Dent Assoc 2012; 92: 23-6; quiz 27-28.

  10. Rateitschak-Plüss EM, Hefti A, Lörtscher R, Thiel G. Initial observation that cyclosporin-A induces gingival enlargement in man. J Clin Periodontol 1983; 10: 237-246.

  11. Sheehy EC, Roberts GJ, Beighton D, O’Brien G. Oral health in children undergoing liver transplantation. Int J Paediatr Dent 2000; 10: 109-119.

  12. Wright G, Welbury RR, Hosey MT. Cyclosporin-induced gingival overgrowth in children. Int J Paediatr Dent 2005; 15: 403-411.

  13. Femiano F, Lanza A, Buonaiuto C, Gombos F, Rullo R, Festa V et al. Oral manifestations of adverse drug reactions: guidelines. J Eur Acad Dermatol Venereol 2008; 22: 681-691.

  14. Pistorius A, Willershausen B, Callaway A. Effects of selected immunouppressive drugs on prostaglandin release, protein synthesis and cell proliferation in human gingival fibroblasts and on the growth of plaque bacteria. Eur J Med Res 2003; 8: 25-32.

  15. Ayanoglou CM, Lesty C. Cyclosporin A-induced gingival overgrowth in the rat: A histological, ultrastructural and histomorphometric evaluation. J Periodontal Res 1999; 34: 7-15.

  16. Lages EJ, Cota LO, Lages EM, Costa JE, Costa FO. Histopathological and morphological alterations of periodontium in rats treated with tacrolimus and cyclosporine. J Int Acad Periodontol 2007; 9: 112-117.

  17. Chang HL, Fu E, Nieh S, Wang SL. Gingival overgrowth induced by different dosages of cyclosporin in rats. Zhonghua Yi Xue Za Zhi (Taipei) 1995; 55: 290-295.

  18. Nishikawa S, Nagata T, Morisaki I, Oka T, Ishida H. Pathogenesis of drug-induced gingival overgrowth. A review of studies in the rat model. J Periodontol 1996; 67: 463-471.

  19. Bolzani G, Della Coletta R, Martelli Junior H, Martelli Junior H, Graner E. Cyclosporin A inhibits production and activity of matrix metalloproteinases by gingival fibroblasts. J Periodontal Res 2000; 35: 51-58.

  20. Kataoka M, Shimizu Y, Kunikiyo K, Asahara Y, Yamashita K, Ninomiya M et al. Cyclosporin A decreases the degradation of type I collagen in rat gingival overgrowth. J Cell Physiol 2000; 182: 351-358.

  21. Chiu HC, Lu YT, Chin YT, Tu HP, Chiang CY, Gau CH et al. Cyclosporine A inhibits the expression of membrane type-I matrix metalloproteinase in gingiva. J Periodontal Res 2009; 44: 338-347.

  22. Cotrim P, Martelli-Junior H, Graner E, Sauk JJ, Coletta RD. Cyclosporin A induces proliferation in human gingival fibroblasts via induction of transforming growth factor-beta1. J Periodontol 2003; 74: 1625-1633.

  23. Gnoatto N, Lotufo RF, Toffoletto O, Marquezini MV. Gene expression of extracellular matrix proteoglycans in human cyclosporin-induced gingival overgrowth. J Periodontol 2003; 74: 1747-1753.

  24. Gagliano N, Moscheni C, Dellavia C, Stabellini G, Ferrario VF, Gioia M. Immunosuppression and gingival overgrowth: gene and protein expression profiles of collagen turnover in FK506-treated human gingival fibroblasts. J Clin Periodontol 2005; 32: 167-173.

  25. Gagliano N, Moscheni C, Dellavia C, Torri C, Stabellini G, Ferrario VF et al. Effect of cyclosporin A on human gingival fibroblast collagen turnover in relation to the development of gingival overgrowth: An in vitro study. Biomed Pharmacother 2004; 58: 231-238.

  26. Bartold PM, Narayanan AS. Biology of the periodontal connective tissues. Chicago: Quintessence; 1998.




2020     |     www.medigraphic.com