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Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán

2012, Number 1

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Rev Invest Clin 2012; 64 (1)

Undifferentiated immunophenotypes and not expression of BCR-ABL can be associated in adult Mestizo Mexican patients with ALL

Vázquez-Franco JE, Reyes-Maldonado E, Montiel-Cervantes LA, Lezama RA

Language: English
References: 38
Page: 32-39
PDF size: 207.81 Kb.


ABSTRACT

Introduction. The BCR-ABL t(9;22)(q34;q11) translocation has been identified as a risk factor in de novo acute lymphoblastic leukemia (ALL), but there are other factors that may influence survival in patients not expressing this translocation. Objective. To associate expression and nonexpression of BCR-ABL with immunophenotype and other clinical features in adult patients with ALL from a Mexican mestizo population. Material and methods. Peripheral blood samples from 35 adult patients with de novo ALL were used to detect BCR-ABL by reverse transcriptase polymerase chain reaction (RT-PCR) as well as immunophenotype by flow cytometry. Results. In the group of BCR-ABL negative patients (74.28%) two subgroups were identified with the immature immunophenotypes CD34+/CD33+ and/or CD13+, and CD10-/CD34+. In the group of BCR-ABL positive patients (25.72%) leukemic blast cells with a more differentiated immunophenotype compared to the BCR-ABL negative group were found. As regards clinical and biological characteristics, we found survival in months to be very similar and a tendency to high initial leukocyte counts in both groups. Conclusions. This is the first study conducted on a Mexican mestizo population to report that BCR-ABL negative patients can present a high frequency of undifferentiated immunophenotypes and must therefore be considered as vulnerable as BCR-ABL positive patients.


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