medigraphic.com

2001, Number 2

<< Back Next >>

Arch Cardiol Mex 2001; 71 (2)

Interaction between NO and 17-β estradiol

Marcelín JG, Ceja OI, Hernández PA, Escalante AB

Language: Spanish
References: 16
Page: 114-120
PDF size: 134.65 Kb.


ABSTRACT

It has been suggested that the low incidence of cardiovascular diseases in premenopausal women, compared with that in men of the same age, is related to the interaction between the nitric oxide (NO) pathway and estrogens. The aim of the present work was to characterize the mechanism by which 17-β estradiol produces an increment in NO release in cultured endothelial cells. Treatment of cells with 17-β estradiol significantly increased the amount of nitrites delivered into the culture medium, compared with that from cells without estrogenic treatment. This effect was blocked by the antagonist of estrogen receptors, tamoxifen. By Western blot, it was shown that 17-β estradiol significantly increased the amount of eNOS in treated cells, compared with that from their respective control cells. Moreover, the acetylcholine-induced release of nitrites in cells treated with 17-β estradiol was higher than nitrite production induced by the same dose of acetylcholine in control cells. In conclusion, our data underline the physiological role of 17-β estradiol, which promotes the increase in eNOS expression, potentiating the effects of vascular agonists that release nitric oxide, suggesting a cardiovascular protective role by estrogens.


REFERENCES

  1. Sánchez LA, Gómez MJ, Dorantes AL, Rosales JL, Pastelín G, Díaz V, et al: The effect of ovariectomy on depressed contractions to phenylephrine and KCl and increased relaxation to acetylcholine in isolated aortic rings of female compared to male rabbits. Br J Pharmacol 1996; 118: 2017-2022.

  2. Wellman GC, Bonev AD, Nelson MT, Brayden JE: Gender differences in coronary artery diameter involve estrogen, nitric oxide and calcium-dependent potassium chanels. Circ Res 1996; 79(5): 1024-1030.

  3. Bobadilla RA, Henkel CC, Henkel EC, Escalante B, Hong E: Possible involvement of endothelium derived hyperpolarizing factor in vascular responses of abdominal aorta from pregnant rats. Hypertension 1997; 30(3P2): 596-602.

  4. Goetz RM, Morano I, Calovini T, Studer R, Holtz J: Increased expression of endothelial constitutive nitric oxide synthase in rat aorta during pregnancy. Biochem Biophys Res Com 1994; 205(1): 905-910.

  5. Vagonni KE, Shaw CE, Phernetton TM, Meglin BM, Bird IM, Magness RR: Endothelial vasodilator product by uterine and systemic arteries. Ovarian and estrogen effects on NO synthase III. Am J Physiol 1998; 275(5P2): H1845-56.

  6. LeMeur M, Glanville N, Mandel JL: The ovalbumin gene family: Hormonal control of X and Y gene transcription and mRNA accumulation. Cell 1981; 23: 561-571.

  7. McKnight GS, Palmiter RD: Transcriptional regulation of the ovalbumin and conalbumin genes by steroid hormones in chick oviduct. J Biol Chem 1979; 254: 9050-9058.

  8. Means AR, Constock JP, Rosenfeld GC: Ovalbumin messenger RNA of chick oviduct: Partial characterization, estrogen dependence and translation in vitro. Proc Natl Acad Sci 1972; 56: 686-693.

  9. Ceccatelli S, Grandison L, Scott REM, Pfaf DW, Kow Lee M: Estradiol regulation of nitric oxide synthase mRNA’s in rat hypotalamus. Neuroendocrinology 1996; 64: 357-363.

  10. Gryglewsky RJ, Moncada S, Palmer RMJ: Bioassay of prostacyclin and endothelium derived relaxing factor (EDRF) from porcine aortic endothelial cells. 1985. Br J Pharmacol 1997; 120(4): 494-503.

  11. Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR: Analysis of nitrite, nitrate and [15N]-nitrite in biological fluids. Annal Biochem 1982; 126: 131-138.

  12. Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Annal Biochem 1976; 72: 248-254.

  13. Zembowicz A, Tang J, Wu KK: Transcriptional induction of endothelial nitric oxide synthase type III by lysophosphatidylcholine. J Biol Chem 1995; 270(28): 17006-17010.

  14. Simionescu N, Simionescu M: The cardiovascular system. In: Weiss L, Greep RO. “Histology”, 4th ed. New York. McGraw-Hill Inc., 1977; 373-432.

  15. Doerr P, Pirke KM: Regulation of plasma oestrogens in normal adult males. II Response of oestradiol, oestrogens, testosterone and cortisol to dexamethasone and ACTH administration. Acta Endocrinol 1975; 78(3): 531-538.

  16. Power RF, Conneely OM, O’Malley BW. New insights into activation of the steroid hormone receptor superfamily. TIPS 1992; 13(8): 318-323.




2020     |     www.medigraphic.com