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Órgano oficial de difusión de la Federación Mexicana de Patología Clínica, AC y de la Asociación Latinoamericana de Patología Clínica/Medicina de Laboratorio

2004, Number 2

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Rev Mex Patol Clin Med Lab 2004; 51 (2)

Rapid latex agglutination test for antibody to hepatitis B surface antigen: performance study and comparison with enzyme immunoassay

Oviedo SA, Zarzur JA

Language: English
References: 17
Page: 93-96
PDF size: 40.89 Kb.


ABSTRACT

Background and objective: A performance evaluation of a latex agglutination test (LAT) manufactured by the Laboratory of Hemoderivados dependent, of the National University of Cordoba, for antibody to Hepatitis B surface antigen (HBsAb), was carried out and compared with a commercial enzyme immunoassay. Material and methods: Testing 1,320 Cordoba’s National University Blood Bank donor and immunized workers from Hemoderivados Laboratory were tested for the presence and levels of HbsAb with LAT and compared with commercial EIA (ABBOTT LABS, North Chicago, Illinois-USA) and RIA (SORIN/Biomedica-Divisione Diagnostica-Italy). Results: This tests showed 100% sensitivity and comparable specificity (LAT 99.5%, EIA 99.7% among donor samples). Conclusion: We conclude that LAT is a specific and sensitive test for HBsAb; it is simple to perform and does no require sophisticated equipment. Hence, it is suitable for mass screening of blood donors in a third world country like Argentina.


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