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2013, Number 1

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Rev Cuba Endoc 2013; 24 (1)

Micoplasma hominis, Ureaplasma urealyticum and aerobic bacteria present in the semen from men attending infertility service

Rodríguez PBV, Ortiz RC, Santana PF, Domínguez AE, Nurquez GB

Language: Spanish
References: 35
Page:
PDF size: 280.46 Kb.


ABSTRACT

Introduction: human semen infections can alter the sperm quality and be associated to male infertility disorders.
Objectives: to determine the frequency of infections from Micoplasma hominis, Ureaplasma urealyticum and other aerobic bacteria in the semen of men who attended the infertility service, and to identify whether there is some relation between the detected infections and the altered semen quality variables or not.
Methods: a cross-sectional descriptive study was performed to evaluate semen samples from 140 men aged 20 to 45 years, who attended the infertility service at the National Institute of Endocrinology. According to the WHO guidelines, a complete spermiogram including leukocytospermia was performed in order to determine the qualitative and quantitative variables in the semen. The semen samples were cultured in blood agar and in chocolate agar at 37oC under CO2 environment to find out possible aerobic bacteria. To this end, a set of reagents known as Mycoplasma System Plus was used, allowing the culture, the identification, the semi-quantitative count and the antibiogram of urogenital mycoplasms/ureaplasms. The ethical aspects were allowed for; the results were analyzed through percentage estimations and the chi square test.
Results: out of the 140 evaluated semen samples, 58 (41.4 %) showed some infection, 37 of them were caused by Ureaplasma urealyticum (25.7 %), 2 by Micoplasma hominis (1.4 %) and 19 by the aerobic bacteria (13.8 %). When making a comparison of the qualitative and quantitative variables of the semen from infected and non-infected subjects, there were not any statistically significant differences in the evaluated variables of the sperm quality.
Conclusions: the total frequency of infections in the studied sample was relatively high, but was not associated to altered seminal variables.


REFERENCES

  1. Estrategias de salud reproductiva. Infecciones de transmisión sexual, incluido el VIH e infecciones del aparato reproductor. Organización Mundial de la Salud. 2004;5:14.

  2. De Jonge C. Semen analysis: looking for an upgrade in class. Fertil Steril. 2012;97:260-6.

  3. Lorusso F, Palmisano M, Chironna M, Vacca M, Masciandaro P, Bassi E, et al. Impact of chronic viral diseases on semen parameters. Andrologia. 2010;42:121-6.

  4. Didelot M, Diafouka F, Yayo E, Kouadio L, Monnet D, Segondy M. HPV seminal shedding among men seeking fertility evaluation in Abidjan, Ivory Coast. J Clin Virol. 2007;39:153-5.

  5. Tsai C, Chang c, Chen K, Thang L. The cost-effectiveness of syndromic management for male sexually transmitted disease patients with urethral discharge symptoms and genital ulcer disease in Taiwan. Sex Transm Inf. 2008;84:400-4.

  6. Zinzerdorf Y, Kouassi T, Lathro S, Don C, Kouadio L, Loukou G. Ureaplasma urealyticum or Micoplasma hominis infections and semen quality of infertile men in Abidjan. J Reprod Contr. 2008;19:65-72.

  7. Rusz A, Pilatz A, Wagenlehner F, Linn T, Diemer T, Schuppe H, et al. Influence of urogenital infections and inflammation on semen quality and male fertility. World J Urol. 2012;30:23-30.

  8. Kiessling A, Desmarais B, Yin H, Loverde J, Eyre C. Detection and identification of bacterial DNA in semen. Fertil Steril. 2008;90:1744-56.

  9. Frontela M, Amores I, Yepe S, Kourí V, Ferreira R, Mallea L. Detección de Chlamydia trachomatis en muestras de exudado endocervical por la reacción en cadena de la polimerasa. Rev Cubana Endocrinol. 2002;13:133-41.

  10. Pérez M, Padrón R, Más J, Peralta H. Semen analyses in men with asymptomatic genital gonorrhea. Int J Androl. 1982;5:6-10.

  11. Ortiz C, Hechavarría C, Calderín M, Álvarez G, Ortiz Y. Estudio de Chlamydia trachomatis, Ureaplasma urealyticum y Mycoplasma hominis en pacientes infértiles y abortadoras habituales. Rev Cubana Obstet Ginecol. 2010;36:573-84.

  12. Duque de Estrada M. Aislamiento e identificación de Ureaplasma spp y Mycoplasma hominis en muestras endocervicales de pacientes infértiles [tesis para optar por el título de Máster en Atención Integral a la Mujer]. Facultad "Comandante Manuel Fajardo". La Habana, Cuba; 2008.

  13. World Health Organization. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interactions. Cambridge, United Kingdom: Cambridge University Press; 1999.

  14. Pellati D, Mylonakis I, Bertoloni G, Fiore C, Andrisani A, Ambrosini G, et al. Genital tract infections and infertility. Eur J Obstet Gynecol Reprod Biol. 2008;140:3-11.

  15. Friberg J, Gnarpe H. Mycoplasmas in semen from fertile and infertile men. Andrología. 1974;6:45-52.

  16. Wang Y, Liang C, Wu J, Xu C, Qin S, Gao E. Do Ureaplasma urealyticum infections in the genital tract affect semen quality? Asian J Androl. 2006;8:562-8.

  17. Zhang Z, Zhang H, Dong Y, Han R, Dai R, Liu R. Ureaplasma urealyticumin male infertility in Jilin Province, North-east China, and its relationship with sperm morphology. J Int Med Res. 2011;39:33-40.

  18. Knox L, Allan A, Allan M, Edirisinghe R, Stenze L, Lawrence L, et al. Ureaplasma parvum and Ureaplasma urealyticum are detected in semen after washing before assisted reproductive technology procedures. Fertil Steril. 2003;80:921-9.

  19. Andrade-Rocha T. Ureaplasma urealyticum and Mycoplasma hominis in men attending for routine semen analysis. Prevalence, incidence by age and clinical settings, influence on sperm characteristics, relationship with the leukocyte count and clinical value. Urol Int. 2003;71:377-81.

  20. Lozano R, Vivas G, Muñoz M. Mycoplasmas and antibodies anti-chlamydia in semen of infertile men and their relationship with seminal quality and markers of male accessory sex glands. Invest Clin. 2012;53:138-47.

  21. Moretti E, Capitani S, Figura N, Pammolli A, Federico M, Giannerini V. The presence of bacteria species in semen and sperm quality. J Assist Reprod Genet. 2009;26:47-56.

  22. Rodríguez R, Hernández R, Fuster F, Torres A, Prieto P. Genital infection and infertility. Enferm Infecc Microbiol Clin. 2001;19:261-6.

  23. Soromón K, Takashi Y. Association between preterm birth and vaginal colonization by Mycoplasmas in early pregnancy. J Clin Microbiol. 2006;4:51-5.

  24. Diaz G, Herrera M, Giono C, Guerra I. Mycoplasma hominis attaches to and locates intracellulary in human spermatozoa. Hum Reprod. 2006;21:1591-8.

  25. Gdoura R, Kchaou W, Chaari H, Znazen A, Keskes L, Rebai T, Hammami A. Ureaplasma urealyticum, Ureaplasma parvum, Mycoplasma hominis and Mycoplasma genitalium infections and semen quality of infertile men. BMC Infect Diseas. 2007;7:129-37.

  26. Núñez R, Gallego S, García M, Cañero A, Moya B, Peregrín P. Análisis microbiológico del semen de los varones en estudio de infertilidad. Rev Int Androl. 2007;5:206-11.

  27. Martínez E, Camejo M. Prevalencia de infecciones en plasma seminal humano. Medicina. 2007;67(supl):28.

  28. López M, Guerra F. Avances en la interacción entre micoplasmas y espermatozoides de humano. Bioquímica. 2008;33:115-21.

  29. Gdoura R, Kchaou W, Ammar- Keskes L, Chakroun N, Sellemi A, Znazen A, et al. Assessment of Chlamydia trachomatis, Ureaplasma urealyticum, Ureaplasma parvum, Mycoplasma hominis and genitalium, in semen and first void urine specimens of asymtomatic male partners of infertile couples. J Androl. 2008;29:198-206.

  30. Ochsendorf F. Sexually transmitted infections: impact on male fertility. Andrologia. 2008;40:72-5.

  31. De Francesco M, Negrini R, Ravizzola G, Galli P, Manca N. Bacterial species present in the lower male genital tract: a five-year retrospective study. Eur J Contracept Reprod Health Care. 2011;16:47-53.

  32. Pérez M, Padrón R. Frequency of asymptomatic seminal infection in infertile patient and its effects on spermiogram parameter. Arch Urol. 1985;25:7-14.

  33. Pérez M, Padrón R, Más J. Efecto de la infección seminal y su tratamiento en la calidad del semen en hombres infértiles. Rev Cubana Endocrinol. 1989;1:37-42.

  34. Bezold G, Politch J, Kiviat N, Kuypers J, Wolff H, Anderson D. Prevalence of sexually transmissible pathogens in semen from asymptomatic male infertility patients with and without leukocytospermia. Fertil Steril. 2007;87:1087-97.

  35. Lackner J, Agarwal A, Mahfouz R, Plessis S, Georg G. The association between leukocytes and sperm quality is concentration dependent. Reprod Biol Endocrinol. 2010;8:12-25.




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