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Órgano oficial de difusión de la Federación Mexicana de Patología Clínica, AC y de la Asociación Latinoamericana de Patología Clínica/Medicina de Laboratorio

2004, Number 2

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Rev Mex Patol Clin Med Lab 2004; 51 (2)

Advantages of the culture in an automated equipment and the PCR test for the Mycobacterium tuberculosis diagnosis

Fagundo SR, Cerros SMA, Herrera PE

Language: Spanish
References: 17
Page: 70-74
PDF size: 75.64 Kb.


ABSTRACT

Because of tuberculosis recovered importance, the need to find new faster and specific diagnostic tools as an impact measure for disease control, has arisen. Our Laboratory has adopted new technologies as PCR microorganism identification, automated culture and antimycobacterial susceptibility test to achieve tuberculosis diagnosis. The present work shows our experience in evaluating 369 patient’s samples for Mycobacterium detection by automated culture means. Samples came from different places of Mexico country in a total period of eighteen months. By automated culture were isolated 44 positive samples to Mycobacterium in an average time of 21 days each one. This isolates then were submitted to PCR characterization and antimycobacterial susceptibility test against isoniazid, rifampin and ethambutol, the first three drugs of choice for treatment. PCR was positive in 82% of isolates demonstrating the importance of this test in detecting false-positive diagnosis of tuberculosis. Forty three per cent of isolates showed resistance against 2 or 3 of the evaluated drugs (multi-drug resistance).


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