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Patología Revista Latinoamericana

2012, Number 4

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Patol Rev Latinoam 2012; 50 (4)

Investigation of human papilloma virus DNA in the cervixes of women in rural population of Peru

Concha-M R, Árias-Stella JJ, Quiñones D, Bazán M, Iwasaki R, Árias-Stella J

Language: Spanish
References: 15
Page: 266-271
PDF size: 218.26 Kb.


ABSTRACT

This study aims to determine the prevalence of HPV and cervical intraepithelial lesions in 384 women –between the ages of 20 and 64-– from the six regions with the highest percentage of rural inhabitants in Peru according to the National Institute of Statistics and Data Processing (INEI). These regions were Huancavelica, Cajamarca, Huanuco, Amazonas, Apurimac and Puno. The sample size was determined using the following statistical formula: n = Z12 × p × q/d2; n = 384.16.
The cytological diagnosis was based on samples suspended in a liquid preparation which were categorized according to the Bethesda Classification System. A Hybrid Capture 2 (HC2) assay and a PCR GP5/GP6 assay were performed to amplify part of the genomes of several HPV types to determine if the infection was caused by one of more HPV types. The results show that 6.77 % of the women had abnormalities detected in the liquid based cytological studies (ASCUS, LGSIL, HGSIL and AGUS) and 4.4 % of the women had some type of lesion according to the histological analysis (LGSIL, HGSIL, adenocarcinoma or epidermoid carcinoma). In conclusion, we found out that there was HPV DNA in all of the detected lesions, the most common being HPV type 16, which was found in 6 (8.22 %) participants with some type of lesion, followed by a co-infection (HPV 16 and 31) in 4 of the participants (5.48 %).


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