medigraphic.com

2011, Number 5

<< Back Next >>

Rev Med Inst Mex Seguro Soc 2011; 49 (5)

Haemophilus influenzae nasopharyngeal colonization in children

Solórzano-Santos F, Miranda-Novales MG, Leaños-Miranda B, Ortiz-Ocampo LA, Echaniz-Avilés G, Palacios-Saucedo G, Guiscafré-Gallardo H

Language: Spanish
References: 23
Page: 499-502
PDF size: 178.69 Kb.


ABSTRACT

Objective: to determine frequency, serotypes, biotypes and susceptibility to eigth antibiotics in Haemophilus influenzae nasopharyngeal isolates in children under five year old from Mexico City.
Methods: cross-sectional survey including children two months to five years old. A nasopharyngeal sample was taken. Haemophilus influenzae identification, serotyping, biotyping and antimicrobial susceptibility were performed.
Results: a sample of 573 children were included. In 88/573 (15.3 %) H. influenzae was isolated, corresponding in 7/573 (1.2 %) to Hib, 3/573 (0.5 %) to Hi a, c, d, f and 78/573 (13.6 %) to Nontypable Hi. Among Hib carriers, 6 had been received only one or two doses of specific vaccine. Biotype VIII (76.1 %) was the predominant. All isolates were susceptible to the antibiotics, but one Hib strain was resistant to eritromycin.
Conclusions: nontypable Haemophilus influenzae was predominant. Colonization by Hib in children under 5 years old was low (1.2 %), occurring this in children with an incomplete vaccination schedule.


REFERENCES

  1. Berman S. Epidemiology of acute respiratory infections in children of developing countries. Rev Infect Dis 1991;13 (Suppl 6):S454-S62.

  2. Janai H, Stutman HR, Marks MI. Invasive Haemophilus influenzae type B infections: a continuing challenge. Am J Infect Control 1990;18(3):160-166.

  3. Games EJ, Solórzano SF, López PJ, Alvarez HL, Ramírez CF. Etiología de la meningoencefalitis purulenta en pediatría. Implicaciones terapéuticas. Gac Med Mex 1991; 127(4):315-320.

  4. Yaro S, Lourd M. Naccro B, Njanpop-Lafourcade BM, Ouedraogo MS, Traore Y, et al. The epidemiology of Haemophilus influenzae type b meningitis in Burkina Faso. Pediatr Infect Dis J 2006;25(5):415-419.

  5. Johnson NG, Ruggerber JU, Balfour GF, Lee YC, Liddy H, Irving D, et al. Haemophilus influenzae type b reemergence after combination immunization. Emerg Infect Dis 2006;12 (6):937-941.

  6. van der Veen EL, Rovers MM, Leverstein-van Hall MA, Sander EA, Scilder AG. Influence of sampling technique on detection of potential pathogens in the nasopharynx. Arch Otolaryngol Head Neck Surg 2006;132(7):752-755. Disponible en http:// archotol.ama-assn.org/cgi/content/full/132/7/752

  7. Aniansson G, Alm B, Anderson B, Larson P, Nylen O, Peterson H, et al. Nasopharyngeal colonization during the first year of life. J Infect Dis 1992;165(Suppl 1):S38-S42.

  8. Dabernat H, Plisson-Sauné MA, Delmas C, Séguy M, Faucon G, Pélissier R, Carsenti H, et al. Haemophilus influenzae carriage in children attending French day care centers: a molecular epidemiological study. J Clin Microbiol 2003;41(4):1664-1672.Disponible en http://www.ncbi.nlm. nih.gov/pmc/articles/PMC153885/?tool=pubmed

  9. Miranda NMG, Solórzano SF, Velázquez AR, Leaños MB, Villasis KMA, Guiscafré GH. Características de los aislamientos de Haemophilus influenzae de portadores asintomáticos en edad pediátrica y su relación con resistencia a antimicrobianos. Bol Med Hosp Infant Mex 1995;52(3):148-153.

  10. Villaseñor-Sierra A, Herrera-Basto E, Vázquez Salazar P, Arroyo-Moreno JA, Santos Preciado JI. Prevalence of Haemophilus influenzae carrier state in children in Ciudad Nezahualcoyotl, State of Mexico, Mexico. Salud Publica Mex 1996;38(2):87-93.

  11. Franco-Paredes C, Lammoglia L, Hernández I, Santos-Preciado JI. Epidemiology and outcomes of bacterial meningitis in Mexican children: 10-year experience (1993-2003). Int J Infect Dis. 2008;12(4):380-386. Epub 2008 Feb 20.

  12. Campos J, Hernando M, Román F et al. Analysis of invasive Haemophilus influenzae infections after extensive vaccination against H. influenzae type b. J Clin Microbiol 2004; 42(2):524-529. Disponible en http://www.ncbi.nlm.nih.gov/ pmc/articles/PMC344522/?tool=pubmed

  13. Murphy TF, Faden H, Bakaletz LO, Kyd JM, Forsgren A, Campos J, et al. Nontypeable Haemophilus influenzae as a pathogen in children. Pediatr Infect Dis J 2009;28 (1):43-48.

  14. Bronfman M, Guiscafré H, Castro V, Castro R, Gutiérrez G. La medición de la desigualdad. Una estrategia metodológica para el análisis de las características socioeconómicas de la muestra. Arch Invest Med 1988;19(4):351-360. Disponible en http://blog.psico.edu.uy/escueladeverano2011/ files/2011/02/La-medici%C3% B3n-de-la-desigualdad.pdf

  15. National Committee for Clinical Laboratory Standards. Antimicrobial susceptibility test for bacteria that grow aerobically: Approved Standard. Fifth edition. Wayne, PA: National Committee for Clinical Laboratory Standards; 2000. NCCLS document M7-A5.

  16. Rodríguez RS, Mascarenas C, Conde-González CJ, Inostroza J, Villanueva S, Velázquez ME, et al. Serological protection induced by Haemophilus influenzae type b conjugate vaccine in Mexican children: Is a booster dose of the vaccine needed? Clin Vaccine Immunol 2010;17(10):1639-1641. Disponible en http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2953005/ ?tool=pubmed

  17. Romanin V, Chiavetta L, Salvay MC, Chiolo MJ, Regueira M, Barrios A, et al. Vacuna anti-Haemophilus influenzaede tipo b (Hib) en el Calendario Nacional de Argentina: portación nasofaríngea de Hib tras ocho años de su introducción. Arch Pediatr Urug 2009;80(1):51-59. Disponible en http://www.scielo.edu.uy/pdf/adp/v80n1/v80n1a11.pdf

  18. Sekhar S, Chakraborti A, Kumar R. Haemophilus influenzae colonization and its risk factors in children aged <2 years in northern India. Epidemiol Infect 2009;137(2):156-160.

  19. De Maria FT, Lim FJ, Barnisham J, Agers WL, Bick GH. Biotypes of serologically nontypable Haemophilus influenzae isolated from de middle ear and nasopharinge of patients with otitis media with effusion. J Clin Microbiol 1984;20 (6):1102-1104. Disponible en http://www.ncbi.nlm.nih.gov/ pmc/articles/PMC271526/?tool=pubmed

  20. Villaseñor-Sierra A, Santos JI. Outer membrane protein profiles of paired nasopharyngeal and middle ear isolates of nontypable Haemophilus influenzae from Mexican children with acute otitis media. Clin Infect Dis 1999;28(2):267-273.

  21. Dabernat H, Plisson-Saune MA, Delmas C, Seguy M, Faucon G, Pelissier R, et al. Haemophilus influenzae carriage in children attending French day care centers: a molecular epidemiological study. J Clin Microbiol 2003;41(4):1664-1672.

  22. Hare KM, Grimwood K, Leach AJ, Smith-Vaughan H, Torzillo PJ, Morris PS, et al. Respiratory bacterial pathogens in the nasopharynx and lower airways of Australian indigenous children with bronchiectasis. J Pediatr 2010;157(6): 1001-1005.

  23. Dinleyici EC, Yargic ZA. Pneumococcal conjugated vaccine: PHiD-CV. Expert Rev Anti Infect Ther 2009;7 (9):1063-1074.




2020     |     www.medigraphic.com