2012, Number 3
<< Back Next >>
Rev Cubana Med Trop 2012; 64 (3)
Influence of biotic factors on the efficacy of Bacillus thuringiensis var. Israelensis against Aedes aegypti (Diptera: Culicidae)
Corbillón PCO, González RA, Menéndez DZ, Companioni IA, Bruzón ÁRY, Díaz PM, Gato AR
Language: Spanish
References: 14
Page: 235-243
PDF size: 135.79 Kb.
ABSTRACT
Introduction: Bacillus thuringiensis H-14 has shown high efficacy on Aedes aegypti larvae. But a number of factors could affect its effectiveness. Knowing these factors is of vital importance for improving the application parameters in real conditions in order to guarantee the treatment's efficiency and to reduce environmental impact.
Objective: to evaluate the influence of some biotic factors on Bacillus thuringiensis H-14 efficacy against Aedes aegypti (Rockefeller).
Methods: an experimental study was conducted following the WHO guidelines. The influence of the food availability, the larval density and the larval staging was evaluated on laboratory bioassays. A commercial Bacillus thuringiensis H-14 formulation against a reference Aedes aegypti strain was used.
Results: the efficacy of Bacillus thuringiensis H-14 decreased in a linear manner with increasing larval density and development stage. This could be related to food intake and availability of the active principle. Competition in food intake resulted in lower efficacy of Bacillus thuringiensis H-14 applications.
Conclusions: the Bacillus thuringiensis H-14 efficacy was affected for the availability of food, the larval development stage and the larval density, so all this should be borne in mind when applying the biolarvicide in situ.
REFERENCES
Akbar NA, Allende I, Balmaseda A, Coelho IC, da Cunha RV, Datta B, et al. Regarding "Dengue-How Best to Classify It". Clin Infect Dis. 2011;53:563-7.
Montella IR, Martins AJ, Viana-Medeiros PF, Lima JB, Braga IA, Valle D. Insecticide resistance mechanisms of Brazilian Aedes aegypti populations from 2001 to 2004. Am J Trop Med Hyg. 2007;77(3):467-77.
World Health Organization. Global strategic framework for integrated vector management. WHO document WHO/CDS/CPE/PVC/2004.10. Geneva: WHO; 2004. Available in: http://www.paho.org/spanish/gov/ce/ce142-24-s.pdf
Polanczyk PA, de Oliveira M, Batista S. Potential of Bacillus thuringiensis H-14 israelensis Berliner for controlling Aedes aegypti. Rev Saude Pub. 2003;37(6):813-6.
Lacey LA. Bacillus thuringiensis serovariety israelensis and Bacillus sphaericus for mosquito control. J Am Mosq Control Assoc. 2007;23(2 Suppl):133-63.
WHO. Guideline specifications for bacterial larvicides for public health use. Report of the WHO informal consultation. Geneva: WHO/HQ; 1999. Available in: http://www.who.int/whopes/resources/resources_1999/en/index.html
Nayar JK, Knight JW, Ali A, Carlson DB, O'Bryan PD. Laboratory evaluation of biotic and abiotic factors that may influence larvicidal activity of Bacillus thuringiensis serovar. israelensis against two Florida mosquito species. J Am Mosq Control Assoc. 1999;15(1):32-42.
Becker N, Zgomba M, Ludwig M, Petric D, Rettich F. Factors influencing the activity of Bacillus thuringiensis var. israelensis treatments. J Am Mosq Control Assoc. 1992;8(3):285-9.
Mulla MS, Darwazehz HA, Zgomba M. Effect of some environmental factors on the efficacy of Bacillus sphaericus 2362 and Bacillus thuringiensis (H-14) against mosquitoes. Bull Soc Vector Ecol. 1990;15(2):166-75.
Boisvert M, Boisvert J. Effects of Bacillus thuringiensis var. israelensis on target and nontarget. Biocontr Sci Technol. 2000;10:517-61.
Stevens MM, Akhurst RJ, Clifton MA, Hughesc PA. Factors affecting the toxicity of Bacillus thuringiensis var. israelensis and Bacillus sphaericus to fourth instar larvae of Chironomus tepperi (Diptera: Chironomidae). J Invert Pathol. 2004;86:104-10.
Wraight S, Molloy D, Jamnback H, Mccoy P. Effects of temperature and instar on the efficacy of Bacillus thuringiensis var. israelensis and Bacillus sphaericus strain 1593 against Aedes stimulans larvae. J Invert Pathol. 1981;38:78-87.
Lei P, Zhao WM, Yang SY, Zhang JS, Liu LJ. Impact of environmental factors on the toxicity of Bacillus thuringiensis var. israelensis IPS82 to Chironomus kiiensis. J Am Mosq Control Assoc. 2005;21(1):59-63.
Aly C. Feeding behavior of Aedes vexans larvae (Diptera: Culicidae) and its influence on the effectiveness of Bacillus thuringiensis H-14 var. israelensis. Bull Soc Vector Ecol. 1983;8(2):94-100.