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Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

2012, Number 3

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Rev Biomed 2012; 23 (3)

ERIC sequences in Chlamydia trachomatis

Hernández-Cortez C, Majalca-Martínez C, Hernández-Méndez JT, Giono- Cerezo S, Aguilera-Arreola MG, Castro-Escarpulli G

Language: Spanish
References: 17
Page: 87-93
PDF size: 262.80 Kb.


ABSTRACT

Introduction. One of the methods used for the typifi cation and for bacterial genetic diversity study is ERIC-PCR. The presence of enterobacterial repetitive intergenic consensus sequences (ERIC) has been described in most Gram negative bacteria, but their presence in bacteria, such as Chlamydia trachomatis, has not been investigated.
Objective. To search in silico and in vitro for ERIC sequences in the Chlamydia trachomatis genome.
Materials and Methods. In silico, ERIC sequences were searched with the bioinformatics tool called FASTA, downloading the genomes of Chlamydia trachomatis D/uw-3/cx, Chlamydia trachomatis 434/Bu, Chlamydia trachomatis L2b/ UCH-1/proctitis, Chlamydia trachomatis A/har- 13, Chlamydia muridarum Nigg, Chlamydophila pneumoniae TW-183, Aeromonas hydrophila, and Escherichia coli K12 deposited in the NCBI database. in vitro, the ERIC-PCR technique was standardized with strains of Chlamydia trachomatis serovars D, L2, and L3.
Results. Were obtained the number of alignments, the alignment region of each ERIC primer, and the expectation (E) and score (S) values of each genome. Afterwards, the presence of ERIC sequences in strains of Chamydia trachomatis serovars D, L2, and L3 was determined, being 44 °C the optimal alignment temperature in the PCR.
Conclusions. With the obtained results we can suggest that the ERIC sequences are present in the Chlamydia trachomatis genome, if this fi nding is reproduced in all the serovars, a new typing method for this bacterium could establish.


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