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2010, Number 4

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Rev Med Inst Mex Seguro Soc 2010; 48 (4)

Two different techniques for volume blood reduction obtained from umbilical cord

Benítez-Arvizu G, Martínez S, Guerra A, Luna F, Romero Y, Novelo-Garza BA

Language: Spanish
References: 15
Page: 443-446
PDF size: 27.81 Kb.


ABSTRACT

Background: to preserve the umbilical cord blood (UCB), the volume containing the cells must be reduced before freezing, but the quality, quantity and functionality of the cells has to be preserved during this procedure. The aim is to compare the performance of two different techniques for volume reduction.
Methods: a semiautomatic system and an automatic system were compared as two different UCB volume reduction techniques. Total nucleated cell (TNC) counts and viability were measured before and after volume reduction. The CD34+ cell counts also were measure
Results: seventy units of cord blood cells (UCB) were collected. Thirty-three volume reduction procedures were performed by semiautomatic system and thirty seven by automatic system. The volume recovered and the CD34+ count in both techniques was similar. Although the viability differed slightly (1 % higher by Optipress II) were observed
Conclusions: the semiautomatic and automatic techniques are suitable to reduce volumes of UCB units.


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