Bonilla-Delgado J, Rodríguez-Uribe G, Cortés-Malagón EM, Sierra-Martínez M, Acosta-Altamirano G, Gariglio-Vidal P

Language: Spanish
References: 19
Page: 419-423
PDF size: 192.67 Kb.

ABSTRACT

Background: mammals have limited epithelial regeneration capacity. The K6b-E6/E7 mice model has been described as useful for the study of epithelial regeneration. The objective of this study is to compare the expression of E6/E7 oncogenes with those of cell proliferation and apoptosis during epithelization. The hypothesis of this study is that alterations in cell proliferation and apoptosis in K6b-E6/E7 mice will only occur during epithelization.
Methods: deep 2 mm punches were performed in the middle of transgenic and control mice’s ears. A biopsy was collected from the epithelization zone 72-hours and 2-weeks post-injury. Assays for cell proliferation and apoptosis were carried out by immunohistochemistry and TUNEL techniques, respectively. RT-PCR in situ was performed to compare E6/E7 expressions in the areas studied.
Results: transgenic strain K6b-E6/E7 presented more proliferative cells and less apoptotic cells in epithelizated zones. This effect was limited to suprabasal stratum only, and correlates with E6/E7 oncogenes expression. Two weeks post-injure, cell proliferation and apoptosis were similar in both samples as the E6/E7 expression went down.
Conclusion: K6b-E6/E7 mouse model is useful for epithelial regeneration. Its mechanisms should be considered for the treatment of deep wounds.

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