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ISSN 0185-3252 (Print)
Revista de la Asociación Médica del Centro Médico ABC

2004, Number 4

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An Med Asoc Med Hosp ABC 2004; 49 (4)

In vivo comparison between preservation mediums MCB and optisol for corneal transplant

Galán DE, Pérez BAL, Quiroz MH, Carrasco RG, Ruíz SLEl, Orozco BC, Olmedo OG, Baca LO, Chapa DJA

Language: Spanish
References: 20
Page: 166-170
PDF size: 74.24 Kb.


ABSTRACT

A study was effectuated in order to compare the effectiveness of the MCB and optisol corneal preservation mediums. Material and methods: Thirty trasplantations were made using rabbit corneas. Some of this donated corneas were preserved during 96 hours in the MCB medium and the others in the optisol medium. The post-operatory evaluating procedures were realized with biomicroscopy, paquimetry (periodically), and with post-mortem speculate microscopy on the sixth month. Results: The 100% of the selected transplantation remained transparent in optisol and MCB until the sixth postoperatory month. The endothelial cell density decreased from 2,300 « 20 cell/mm2 on fresh tissue to 2,000 « 62 cells/mm2 in optisol, with 70% hexagonality in comparison with the MCB results which are the following: The cell density decreased to 1,620 « 38 cell/mm2, and the hexagonality was of the 57%. This results were on the sixth postoperatory month. Conclusion: The results indicate that MCB fulfils the requirements of and ideal medium, due to its simplicity, economy (approximately one third of the cost of the Optisol) and keeps the endothelial viability for 96 hours before the transplantation. It also keeps the tissue thin, allowing an effective quirurgical manipulation, as well as it’s storage for short periods in the eye banks.


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