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2010, Number 4

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Rev Mex Med Repro 2010; 2.3 (4)

Results of three-year-experience on vitrification to oocyte bank

Cuneo PS, Sánchez GS, Cubillos GS

Language: Spanish
References: 23
Page: 96-100
PDF size: 69.38 Kb.


ABSTRACT

Objective: To show that oocyte vitrification and the sequential culture are a safe combination to have a viable success in an oocyte donation bank program.
Material and method: Thirty cycles of oocytes donors (310 oocytes) were frozen by vitrification. We studied 16 patient’s cycles of thawed oocytes receptors, from May 2006 to December 2009. As a control group we included 48 patient’s cycles of fresh oocyte receptors (600 oocytes). Oocytes were vitrified with the Cryotop method. One hundred fifty-four oocytes were thawed, and a mean of 9.6 metaphase II oocytes were assigned for each recipient. The survived oocytes were fertilized by ICSI and cultured until day 5 or 6 according to embryo development. Mann-Whitney U-tests were carried out. Significance was defined as p ‹ 0.05.
Results: In the vitrified oocytes group, pregnancy, implantation and miscarriage rates were: 66.6%, 316% and 22.2%, respectively. In the fresh oocyte group, the same rates were: 70.5%, 36.1% and 13%, respectively. No statistical differences were found between both groups.
Conclusions: Our excellent clinical outcome indicates the safety to use the vitrification to create oocyte donation bank programs for human reproductive purposes. Sequential culture is a viable strategy to consider selecting embryos with a high potential implantation to transfer. More studies should be done to confirm these results.


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