Maldonado I, Lazcano J, López P, Bermúdez A, Dabbah J, Moreno D, Cedillo FJ, Ruiz S, Gaytán MJE

Language: Spanish
References: 16
Page: 84-87
PDF size: 62.10 Kb.

ABSTRACT

Different reports throughout the world and our own experience have demonstrated that the vitrification technology by the cryotop method as an alternative for the cryopreservation of human oocytes has been successful, in terms of survival, fertilization, embryo development, pregnancy, etc., using ISCI as the unique method for thawed oocyte insemination. This article reports the first results of a project designed to implement the conventional IVF in those patients whose sperm quality does not make necessary the application of the ICSI method for insemination and to try to reduce, as much as possible, the excessive use of intracytoplasmic microinjection in cycles where it is not necessary.

REFERENCES

1. Cobo A, Kuwayama M, Perez S, et al. In-vitro and clinical evaluation of oocytes vitrification for egg donation programs. Lyon: Scientific programme Overview, 2004.

2. Kuwayama M. Highly efficient vitrification for cryopreservation of human oocytes and embryos: the Cryotop method. Theriogenology 2007;1:73-80.

3. Chian R, Kuwayama M, Tan L, et al. High survival rate of bovine oocytes matured in vitro following vitrification. J Reprod Dev 2004;50:685-696.

4. Glujovsky D, Pesce R, Fiszbajn G, et al. Endometrial preparation for women undergoing embryo transfer with frozen embryos or embryos derived from donor oocytes. Cochrane Database Syst Rev 2010;1:CD006359.

5. Rosenwaks Z. Donor eggs: Their application in modern reproductive technologies. Fertil Steril 1987;47:895-909.

6. Sauer M, Paulson R, Lobo R. Pregnancy after age 50: application of oocyte donation to women after natural menopause. Lancet 1993;341:321-323.

7. Remohi J, Vidal A, Pellicer A. Oocyte donation in low responders to conventional ovarian stimulation for in vitro fertilization. Fertil Steril 1993;59:1208-1215.

8. Chen C. Pregnancy after human cryopreservation. Lancet 1986;1:884-886.

9. Kuwayama M. Highly efficient vitrification for cryopreservation of human oocytes and embryos: the Cryotop method. Theriogenology 2007;67:73-80.

10. Kuwayama M, Vajta G, Kato O, et al. Highly efficient vitrification method for cryopreservation of human oocytes. Reproductive Biomed Online 2005;11:300-308.

11. Kuwayama M, Vajta G, Ieda S, et al. Comparison of open and closed methods for vitrification of human embryos and the elimination of potential contamination. Reproductive Biomed Online 2005;11:608-614.

12. Shaw J, Oranratnacha A, Trouson A. Fundamental cryobiology of human mammalian oocytes and ovarian tissue. Theriogenology 2000;53:59-72.

13. Imoedemhe D, Sigue A. Survival of human oocytes cryopreserved with or without the cummulus in 1,2 propanediol. J Assist Reprod Genet 1992;9:323-327.

14. Ruvalcaba L, Martínez R, Cuneo S, et al. Improving donor programs with an oocyte bank using vitrification. Fertil Steril 2005;84(Suppl. 1):S70.

15. Cobo A, Bellver J, Domingo J, et al. New options in assisted reproduction technology: the cryotop method of oocyte vitrification. Reproductive Biomed Online 2008;17:68-72.

16. Antinori M, Licata E, Dani G, et al. Cryotop vitrification of human oocytes results in high survival rate and healthy deliveries. Reproductive Biomed Online 2007;14:72-79.