Arencibia DF, Rosario LA, Suárez YE, Delgado L

Language: Spanish
References: 24
Page: 21-26
PDF size: 150.68 Kb.

ABSTRACT

This research aimed at comparing Balb/c mice and Sprague Dawley rats as biomodel in the head sperm morphology assay, keeping in mind the frequency in anomalous forms in the sperm head basal and induced with cyclophosphamide (CF). Twenty animals/group were used of Balb/c mice line and Sprague Dawley (SD) rats, administered during 35 days in the mouse and 52 days in the rat. Using a negative control group (not administered), two substance-vehicle controls and positive control CF administered with 50 mg/kg, by intraperitoneal route during five consecutives days. The best experimental biomodel turns out to be the Balb/c mice differing significantly from the results obtained in the SD rats, keeping in mind the spontaneous and induced values in the spermatic concentration and the frequency of anomalous epididymis sperms. This study will allow to use mice species with more efficiency in the genotoxic and preclinical toxicology fertility evaluation of drugs, vaccine and other products.

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