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2012, Number 2

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Enf Infec Microbiol 2012; 32 (2)

Migratory properties of dendritic cells and degree of infectivity by infection with Leishmania (L) mexicana in the murine model of tolerance

Cisneros GLE, Pereira RGJ, Aguin MVJ

Language: Spanish
References: 28
Page: 46-54
PDF size: 319.65 Kb.


ABSTRACT

In the murine model of tolerance to infection by Leishmania (L) mexicana, dendritic cells (CDs) are critical in this response, this may be due to an alteration in their migratory prowess. This property may be affected by various stimuli (parasite, chemokines or cytokines). In our research we study the migration and infectivity of CDs of mice BALB/c healthy (CDsS), infected in vivo (CDsI) and in vitro (CDsP).
Methodology. In migration, were isolated CDsS, CDsI and CDsP with Leishmania (L) mexicana, placed in a chemotaxis chamber, compared RANTES, TNF, and supernatant of parasite. For intracellular infectivity were preincubated promastigotes of Leishmania (L) mexicana with fluorochrome, and then assessed the degree of infection by fluorescence flow cytometry.
Results. The rate of migration (IM) of CDsS, was significantly higher when compared with CDsI and CDsP. The IM of CDsS and CDsP in the presence of RANTES and TNF, was significantly greater than that of CDsI, this migration depends on the concentration of such cytokines.
Discussion. The results suggest that the microenvironment in which the CDs is exposed to the stimulus is critical for the migratory behavior of that cell. In terms of infectivity, the previous contact with the parasite reduces its ability to capture and process the antigen (Ag), establishing a state of tolerance.
Conclusion. The migration of CDs depends on the microenvironment, the intensity and type of stimulus, and previous contact with antigen. The infectivity depends of the previous tolerogenic state of the CDs.


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