Dopico JR, Álvarez M, Juvier AI, Trujillo J, Reyes G, Pico MC, Casadelvalle I, Giraldino I

Language: Spanish
References: 14
Page: 10-15
PDF size: 210.69 Kb.

ABSTRACT

Avian antibodies (IgY) display some advantages in relation to the mammalian IgG antibodies due to its easy procedure for generation and purification and besides for its lower production cost. The objective of this work was to analyze the feasibility of binding IgY antibodies to polystyrene particles and to evaluate its performance in latex-agglutination tests in slide. For this purpose, the detection of hepatitis B surface antigen (HBsAg) was used as a model. Leghorn hens were immunized with the active pharmaceutical ingredient (MPA) used in the manufacturing of the Cuban recombinant vaccine Heberbiovac HB. The obtained antibodies were purified and used in the preparation of the latex particles (0.8 μm) for the detection of the HBsAg by agglutination. The performance of the test was evaluated with 50 serum samples against similar commercial reagent. The results obtained were satisfactory and demonstrated the feasibility of binding such avian antibodies to latex particles. These results open a perspective to the use of this type of assay for the rapid diagnostic of many diseases.

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