Pérez-Olvera O, Arellano BS, Rodríguez MHA

Language: Spanish
References: 21
Page: 63-71
PDF size: 531.08 Kb.

ABSTRACT

In Medicine, a large number of investigations require a morphometric analysis of an organ, tissue or cell and/or the study of their internal composition. Stereological methods are the result of mathematical reasoning explained in applicable formulas designed to determine parameters such as volume density, surface density, numerical density and length density, from bidimensional images. Changes in cellular function, for example, are frequently related to variations in the size of cells and nuclei, or to changes in the number of specific cellular organelles involved in the process. A brief review of the physical-mathematical principles that gave rise to the classic stereological methods is presented. We comment upon a series of conceptions on stereological principles over the past few decades in order to prevent under or overestimations in the appraisal of the results that the method allows. The stereological methods more frequently used are also examined. Currently software development simplifies the application of morphometric studies, whose main advantage is that they allow quantifications in a short time.

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