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Órgano oficial de difusión de la Federación Mexicana de Patología Clínica, AC y de la Asociación Latinoamericana de Patología Clínica/Medicina de Laboratorio

2012, Number 2

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Rev Mex Patol Clin Med Lab 2012; 59 (2)

TLR2 and TLR4 expression on CD14+ cells from preterm infants with risk factors for neonatal sepsis development

Mancilla-Ramírez J, Nava K, Galindo-Sevilla N, Segura-Cervantes E, García-Carrillo L

Language: English
References: 32
Page: 107-113
PDF size: 57.56 Kb.


ABSTRACT

Introduction: Neonatal sepsis (NS) is a systemic infection exhibiting 25% lethality among infected children, and it poses a higher risk in preterm than in full-term neonates. Routine microbiological culture, hematological quantification, IL-6, IL-8, C-reactive protein and procalcitonin determinations are the most commonly used tests to diagnose sepsis. However, these methods have substantial limitations due to the associated variation, low degree of sensitivity and long period of time required for execution. These factors decrease the efficiency of an early diagnosis. Toll-like receptors are involved in native immunity recognition and the stimulation of antimicrobial mechanisms. Therefore, they may be altered early during the systemic infection and may be useful in the diagnosis of sepsis. Methods: In this work, we analyze the expression of TLR2 and TLR4 on CD14+ cells as early markers for sepsis in preterm neonates. Results: Our results show that the percentage of CD14int cells and their TLR2 expression were significantly altered in preterm infants with diagnosed infections and were not altered in the systemic inflammatory response syndrome (SIRS) patients. Discussion: These results suggest that TLR2 screening could be a helpful diagnostic test for bacterial infection in preterm infants.


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