Rincón MCA, Castaño OJC, Ríos VE

Language: Spanish
References: 18
Page: 160-171
PDF size: 169.94 Kb.

ABSTRACT

Introduction: Acmella ciliata (Kunth) Cass. is a native weed of northern South America known by its aliphatic alkamide content and used as an anesthetic and analgesic for toothache and sore throat.
Objective: to obtain, to analyze and to evaluate the biological activities of essential oils from Acmella ciliata (Kunth) Cass flowers and leaves.
Methods: Acmella ciliata essential oils were obtained by hydrodistillation and microwave-assisted hydrodistillation and analyzed by gas chromatography coupled to mass spectrometry. The biological activity of both essential oils was determined by using the modified agar-well diffusion assay, evaluating their activities against five bacteria and one fungus, along with tests of toxicity in Artemia salina (Lethal Dose 50).
Results: microwave-assisted hydrodistillation was the technique with the best performances in the extraction of Acmella ciliata. essential oils. Their volatile fractions contain a high proportion of sesquiterpenes such as β-trans-caryophyllene, the major component. Essential oils at two different concentrations (25 mg/mL and 15 mg/mL) showed a strong antimicrobial activity against gram-positive bacteria Staphylococcus aureus and Staphylococcus epidermidis, in addition they exhibited low toxicity against Artemia salina, presenting mean lethal doses of 176, 156 and 100,104 ppm for the essential oils from leaves and flowers, respectively.
Conclusions: Acmella ciliata essential oils are products with high content of terpenoids and marked antimicrobial activity against gram-positive bacteria (Staphylococcus aureus and Staphylococcus epidermidis) and low toxicity against Artemia salina.

REFERENCES

1. Chung K, Kono Y, Wang C, Peng C. Notes on Acmella (Asteraceae: Heliantheae) in Taiwan. Bot Stud. 2008;49:73-82.

2. Vélez MC, Agudelo CA, Pinto DM. Monografías de la flora andina: Flora arvense de la región cafetera centro-Andina de Colombia tomo I. Armenia (Colombia): Universidad del Quindío;1998.

3. Molina J, García A. Alcamidas en plantas: distribución e importancia. Avance Perspectiva. 2001;20:377-87.

4. Sarnaglia T, Moret C, Felix AP, Holanda MC, Salazar O, Kaplan M. Análise do Óleo Essencial dos Diferentes Órgãos de Acmella ciliata Kunth (Asteraceae). Rev Bras Bioci. 2007;5(2):1128-30.

5. Keipert R. Acmella ciliata (H.B.K.) Cassini. Phytochemische und enzymatische Untersuchungen, galenische Präformulierungen [Tesis para Doctor en Ciencias]. Universidad Libre de Berlín;2009.

6. Ríos E, Giraldo GA, Leon DF, Moreno A. Evaluación del perfil de compuestos volátiles de los rizomas de Curcuma longa l. cultivada en el departamento del Quindío-Colombia. Rev Invest Universidad Quindío. 2008;18:32-7.

7. Henao J, Muñoz LJ. Evaluación de la actividad antimicrobiana de los extractos y del aceite esencial obtenidos de Lippia origanoides H.B.K. cultivada en el Quindío [Trabajo para optar por el título de Químico]. Universidad del Quindío;2009.

8. Rojas J, García A, López A. Evaluación de dos metodologías para determinar la actividad antimicrobiana de plantas medicinales. Boletín Latinoamericano y del Caribe de plantas medicinales y aromáticas. 2005;4(2):28-32.

9. Golmakani MT, Rezei K. Comparison of microwave-assisted hydrodistillation with the traditional hydrodistillation method in the extraction of essential oils from Thymus vulgaris L. Food Chem. 2008;109(84):925-30.

10. Muñoz D, Voguel H, Razmilic I. Variación de compuestos químicos en hojas de poblaciones de Drimys spp. (Magnoliophyta: Winteraceae) en Chile. Rev Chil Hist Nat. 2004;77:43-50.

11. Rajalakashmi R. Cytological and phytochemical investigations in some medicinal plants of asteraceae [Trabajo para optar por el título de Doctor of Philosophy en Botánica]. Universidad Mahatma Gandhi;2001.

12. Martin R, Becker H. Amides and other constituents from Acmella ciliata. Phytochemistry. 1985;24(10):295-300.

13. Ahmad I, Aqil F, Owais M. Modern Phytomedicine: turning medicinal plants into drugs. Weinheim: WILEY-VCH Verlag GmbH & Co. KgaA;2006.

14. Murphy M. Plant Products as antimicrobial agents. Clin Microbiol Rev. 1999;12:564-82.

15. Maguna F, Romero A, Garro O, Okulik N. Actividad antimicrobiana de un grupode terpenoides. Facultad de Agroindustrias, UNNE, Argentina[Comunicaciones Científicas Tecnológicas en internet]. 2006[citado 23 Sep 2011];E(57). Disponible en: http://www.unne.edu.ar/Web/cyt/cyt2006/08-Exactas/2006-E-057.pdf

16. Burt S. Essential oils: their antibacterial properties and potential applications in foods-a review. Int J Food Microbiol. 2004;94:223-53.

17. Rany S, Murty S. Antifungal potential of flower head extract of Spilanthes acmella Linn. Afr J Biomed Res. 2006,9:67-9.

18. Flores E, Velasco A, Irahola P, Gimenez A. Aceites esenciales con actividad citotóxica como indicador de propiedades insecticidas. Biofarbo.1999;7(35):35-7.