medigraphic.com
ISSN 1561-3054 (Electronic)

2012, Number 1

<< Back Next >>

Rev Cubana Med Trop 2012; 64 (1)

Preservation of high risk fungal cultures of Histoplasma and Cryptococcus

Fernández ACM, Díaz SLA, Illnait ZMT, Aragonés LC, Martínez MG, Perurena LMR

Language: Spanish
References: 20
Page: 49-54
PDF size: 90.82 Kb.


ABSTRACT

Introduction: culture collections are responsible for providing the microbial resources for development of biological sciences. Storage in distilled water is one of the easiest and least expensive method for long-term fungal preservation. Objective: to evaluate the usefulness of this preservation method in fungal culture of Histoplasma and Cryptococcus. Methods: the preservation condition of the highest biological risk species from Histoplasma y Cryptococcus genera, included in the fungal culture collection of “Pedro Kourí” Institute of Tropical Medicine in Havana, was evaluated in this study. One hundred and two strains stored in distilled water, 92 % of which had been preserved for more than 10 years, were analyzed. Results: the percentages of recovered strains from H. capsulatum, C. neoformans and C. gattii were 64.3 %; 79.1 % and 100 % respectively. This method of preservation proved to be satisfactory for fungal culture in labs with limited financial resources. A web-based database with interesting information about the collection was made. The importance of strict compliance with the biosafety measures in these collections, particularly with high risk pathogens. Conclusions: preservation of fungal cultures in distilled water is a very useful method for laboratories with limited resources. Culture collections should be assumed as an essential activity in order to solve increasing challenges in the development of biomedical sciences.


REFERENCES

  1. Smith D. Culture collections over the world. Int Microbiol. 2003;6 (2):95-100.

  2. Fernández Andreu CM, Martínez Machín G, Perurena Lancha MR, Illnait Zaragozí MT, Valdés Hernández I. La colección de cultivos de hongos del Instituto de Medicina Tropical “Pedro Kourí”: funciones y retos. Rev Cubana Med Trop. 2005;57(3):214-8.

  3. Hartung de Capriles C, Mata S, Middelveen M. Preservation of fungi in water (Castellani): 20 years. Mycopathologia.1989;106 (2):73-9.

  4. Rodrigues EG, Lírio VS, Lacaz CS. Preservação de fungos e actinomicetos de interesse médico em água destilada. Rev Inst Med Trop São Paulo. 1992;34 (2):159-65.

  5. Pérez C, Mata-Essayag S, Hartung de Capriles C, Roselló A, Colella MT, Olaizola C, et al. Mantenimiento de Cryptococcus sp. con el método de Castellani. Rev Soc Ven Microbiol. 2003;23 (2):153-7.

  6. Diogo HC, Sarpieri A, Pires MC. Preservação de fungos em água destilada. An Bras Dermatol. 2005;80 (6):591-4.

  7. Borman AM, Szekely A, Campbell CK, Johnson EM. Evaluation of the viability of pathogenic filamentous fungi after prolonged storage in sterile water and review of recent published studies on storage methods. Mycopathologia. 2006;161 (6):361-8.

  8. De Hoog GS, Guarro J, Gené J, Figueras MJ. Atlas of clinical fungi. 3rd ed (version piloto in CD Rom). Utrech-/Reus: Centraalbureau voor Schimmelcultures/ Universitat Rovira i Virgili; 2009. p. 10-1.

  9. Nakasone KK, Peterson SW, Jong SC. Preservation and distribution of fungal cultures. In: Mueller GM, Bills GF, Foster MS, editors. Biodiversity of fungi. Inventory and monitoring methods. Amsterdam: Elsevier Academic Press; 2004. p. 37-47.

  10. Lima RF, Borba CM. Viability, morphological characteristics and dimorphic ability of fungi preserved by different methods. Rev Iberoam Micol. 2001;18 (4):191-6.

  11. Pasarell L, Mc Ginnis MR. Viability of fungal cultures maintained at -70 ºC. J Clin Microbiol. 1992;30 (4):1000-4.

  12. Borrell N, Mesquida X, Alomar P. Normas de seguridad. En: Pemán J, Martín-Mazuelos E, Rubio-Calvo MC, editores. Guía práctica en identificación y diagnóstico en micología clínica. Valencia: Ed. Revista Iberoamericana de Micología; 2001. p. 17.1-13. ISBN: 84-607-3050-6

  13. US Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiology and Biomedical Laboratories (BMBL). 5th ed. Washington: US Government Printing Office; 2007 [cited Aug 3 2011]. Disponible en: http://www.cdc.gov/od/ohs/biosfty/bmbl5/bmbl5toc.htm

  14. Fernández Llanes R, Fernández Andreu CM, Fuentes González O. Riesgo biológico asociado con trabajos de campo: informe de dos casos de histoplasmosis. Rev Cubana Med Trop. 1987;39(1):61-7.

  15. Casadevall A, Pirofski LA. The weapon potencial of human pathogenic fungi. Med Mycol. 2006;44 (8):689-96.

  16. Sewell DL. Laboratory-associated infections and biosafety. Clin Microbiol Rev. 1995;8 (3):389-405.

  17. Buitrago MJ, Gonzalo-Jiménez N, Navarro M, Rodríguez- Tudela JL, Cuenca-Estrella M. A case of primary cutaneous histoplasmosis in the laboratory. Mycoses. 2011, doi:10.1111/j.1439-0507.2010.02003.x

  18. Glaser JB, Gordon A. Inoculation of Cryptococcus without transmission of acquired immune deficiency syndrome. N Engl J Med. 1985;313 (4):266.

  19. Casadevall A, Mukherjee J, Yuan R, Perfect J. Management of injuries caused by Cryptococcus neoformans-contaminated needles. Clin Infect Dis. 1994;19 (5):951-3.

  20. Padhye AA, Bennett JE, McGinnis MR, Sigler L, Flisa A, Salkin IF. Biosafety considerations in handling medically important fungi. Med Mycol. 1998;36(Suppl. 1):258-65.




2020     |     www.medigraphic.com