Arencibia ADF, Rosario FLA, Suárez FYE, Delgado RL

Language: Spanish
References: 24
Page: 40-48
PDF size: 73.19 Kb.

ABSTRACT

Objectives: to compare fetuses from Balb/c mice and Sprague Dawley rats as biomodels in transplacental micronuclei assay and to determine the spontaneous and induced frequency to link the genotoxic and reproductive effect.
Methods: three experimental groups by species were formed: a negative control (simulation), NaCl (0.9 %) solvent control and 50 mg/kg cyclophosphamide. All the groups were intraperitoneally administered at 14th, 15th and 16th days of gestation, and 24 h after the last inoculation in mice and 48 h in rats, it proceeded to perform euthanasia in the pregnant animals to obtain the fetal liver samples.
Results: the fetuses from Sprague Dawley exhibited smaller cytotoxicity and genotoxicity indexes, and the lowest endogenous micronucleus results. The best results of the cytotoxicity and genotoxicity induction for the high micronucleus formation with cyclophosphamide were found in Sprague Dawley rat fetuses, being more susceptible to the genotoxic damage by this mutagen. The clastogenic transplacental power of cyclophosphamide was confirmed whereas this genotoxicity assay was linked to reproduction toxicology.
Conclusions: these results suggest that the Sprague Dawley rats fetuses could be better used as biomodels in this assay when cyclophosphamide is employed as positive control through the way of administration and the studied dosage. It could be similarly used in the evaluation of new antigenotoxic drugs with antigenotoxic effect through transplacental administration

REFERENCES

1. Hayashi M, Tice RR, MacGregor JT, Anderson D, Blakey DH, Kirsh-Volders M, et al. In vivo, Rodent Erythrocyte Micronucleus Assay. Mutation Res. 1994;312(2):293-304.

2. Organisation for Economic Co-operation and DevelopmenT. Genetic Toxicology: In vivo Mammalian Chromosome Aberration Test, in bone. marrow cells. Anexo B11. In: OECD. Guideline for the testing of chemical. Directrices de OCDE TG 475. Paris: OECD Publishing; 1997. p. 5-6.

3. Arencibia DF, Rosario LA, Morffi J, Curveco D. Desarrollo y estandarización de la técnica en tres ensayos de genotoxicidad. Retel. 2009;25(3):22-38.

4. Schmid W. The micronucleus test. Mutation Res. 1975;31:9-15.

5. Gollapudi B, McFadden LG. Sample size for the estimation of polychromatic to normochromatic erythrocyte ratio in the bone marrow micronucleus test. Mutation Res. 2000;354(2):97-9.

6. Gámez R, Fernández I, Acosta PC, Alemán C, Rodeiro I, Rodríguez M. Frecuencia espontánea e inducida de micronúcleos en médula ósea y mutaciones letales dominantes en ratones NMRI. Rev CENIC. 2000;31(3):211-6.

7. Porter A, Singh G. Trasplancental teratogenesis and mutagenesis in mouse fetuses treated with Cyclophosphamide. Teratogenesis Carcinogenesis Mutagenesi. 1988;8(4):191-203.

8. Fraga JR, Domínguez Y, Friman M, González SB, Somoza AD, Pérez C. Evaluación genotóxica de la vacuna antileptospirósica vax-SPIRAL® empleando el ensayo de micronúcleos trasplacentarios. Anuario Toxicol. 2001;1(1):35-9.

9. Arencibia DF, Rosario LA, Rodríguez Y, Martin Y, Díaz D. Frecuencia espontánea e inducida de anomalías en la morfología de la cabeza del espermatozoide y micronúcleos en médula ósea de ratones Balb/c y OF-1. Retel. 2009;24(2):7-29.

10. Arencibia DF, Rosario LA, Suárez YE, Vidal A. Evaluación y comparación de cuatro líneas de ratones como biomodelo en los ensayos de genotoxicidad. Biocyt. 2011;4(14):240-6.

11. Arencibia DF, Rosario LA, Suárez YE, Vidal A. Evaluación y comparación de tres líneas de ratas como biomodelos en ensayos de genotoxicidad. Animales de Laboratorios. 2011;51(3):57-65.

12. Arencibia DF, Rosario LA, Suárez YE, Vidal A. Carta al Editor: Evaluación y comparación entre tres líneas de ratas como biomodelos en cuatro ensayos de genotoxicidad. Retel. 2011;35(2):16-24.

13. Olfert ED, Cross BM, McWilliam DVM, McWilliam AA (Eds.). Canadian Council on Animal Care (CCAC). Guidelines for the use of animals in Psychology. Ottawa: Bradda Printing Services Inc; 1997. p. 155-62.

14. Arencibia DF, Rosario LA, Suárez YE. Ensayo de micronúcleos transplacentarios en roedores, una buena opción en toxicología experimental. Retel. 2010;33(4):33-41.

15. Adler ID, Bootman J, Favor J, Hook G, Schriever G, Welzl G, et al. Recommendations for statistical designs of in vivo mutagenicity tests with regard to subsequent statistical analysis. Mutation Res. 1998;417:19-30.

16. Matuo R, Oliveira RJ, Silva AF, Mantovani MS, Ribeiro LR. Anticlastogenic Activity of Aqueous Extract of Agaricus blazei in Drug-Metabolizing Cells (HTCs) During Cell Cycle. Toxic Mechan Meth. 2007;17(3):147-52.

17. Meintieres S, Nesslany F, Pallardy M, Marzin D. Detection of ghost cells in the standard alkaline comet assay is not a good measure for the apoptosis. Envir Mol Mut. 2003;41:260-9.

18. Das UB, Mallick M, Debnath JM, Ghosh D. Protective effect of ascorbic acid on cyclophosphamide-induced testicular gametogenic and androgenic disorders in male rats. Asian J Androl. 2002;4:201-7.

19. Rezvanfar MA, Sadrkhanlou RA, Ahmadi A, Shojaei H, Rezvanfar MA. Protection of cyclophosphamide-induced toxicity in reproductive tract histology, sperm characteristics, and DNA damage by an herbal source; evidence for role of free-radical toxic stress. Human & Exp Toxicol. 2008;27:901-10.

20. Arencibia DF, Vidal A, Rosario LA, Suárez YE, Delgado L. Biomodelos para la inducción de micronúcleos en células de la médula ósea por ciclofosfamida y bleomicina. VacciMonitor. 2011;20(1):28-33.

21. Arencibia DF, Rosario LA. Evaluación de ratas Sprague Dawley como biomodelos experimentales en el ensayo de micronúcleos y de aberraciones cromosómicas en células de la médula ósea. Rev Vet Argent. 2010;27(264):1-13.

22. Bell DR, Plant NJ, Rider CG, Na L, Brown S. Species-speciûc induction of cytochrome P-450 4A RNAs: PCR cloning of partial guinea-pig, human and mouse CYP4A cDNAs. Biochem J. 1993;294:173-80.

23. Amri H, Batt A, Siest G. Comparison of cytochrome P-450 content and activities in liver microsomes of seven species including man. Xenobiotica. 1986;16:351-8.

24. Arencibia DF, Rosario LA. Evaluación de ratas Sprague Dawley como biomodelo para detectar daño en el ADN de leucocitos de sangre periférica y células hepáticas, mediante el ensayo Cometa. ARS Pharmaceutica. 2010;51(1):49-56.