Díaz-Elizondo G, Hernández-Mata A, Hernández-Cueto D, Baay-Guzmán GJ, Huerta-Yépez S

Language: Spanish
References: 23
Page: 203-212
PDF size: 641.00 Kb.

ABSTRACT

remodelling. The mechanisms implicated in the pathogenesis of this disease remain unclear. Several studies have shown that TGF-β plays an important role in the pathogenesis of asthma. In addition, the polymorphism of the TGF-β promoter region results in the overexpression of TGF-β via regulation of the transcription factor Yin-Yang-1 (YY1). It is has recently been demonstrated that YY1 may be involved in the pathogenesis of asthma by the regulation of IL-4 and IL-10. The aim of this study was to evaluate the association between the YY1 and TGF-β expression levels in a murine model of lung allergic inflammation. Methods. In this study we used a lung allergic inflammatory murine model with different severity degrees. Tissue microarray technology and immunohistochemistry were used to evaluate YY1 and TGF-β expression. The density expression was measured by quantitative methods using specific software. Results. Expression of both proteins correlated with the degrees of severity of lung allergic inflammation. A similar result was observed with mucus production. Conclusions. These results corroborate the role of YY1 and TGF-β in the pathogenesis of this disease.

REFERENCES

1. Blumenthal M. The immunopathology and genetics of asthma. Minn Med 2004;87:53-56.

2. Anandan C, Nurmatov U, van Schayck OC, Sheikh A. Is the prevalence of asthma declining? Systematic review of epidemiological studies. Allergy 2010;65:152-167.

3. Carrada BT. Asthma: prevalence, pathogenesis and perspectives on new treatments. Rev Alerg Mex 2002;49:87-94.

4. Karaman O, Arli O, Uzuner N, Islekel H, Babayigit A, Olmez D, et al. The effectiveness of asthma therapy alternatives and evaluating the effectivity of asthma therapy by interleukin-13 and interferon gamma levels in children. Allergy Asthma Proc 2007;28:204-209.

5. Pedersen S. From asthma severity to control: a shift in clinical practice. Prim Care Respir J 2010;19:3-9.

6. Górska K, Krenke R, Kosciuch J, Korczynski P, Zukowska M, Domagala-Kulawik J, et al. Relationship between airway inflammation and remodeling in patients with asthma and chronic obstructive pulmonary disease. Eur J Med Res 2009;14(suppl 4):90-96.

7. Beasley R, Roche WR, Roberts JA, Holgate ST. Cellular events in the bronchi in mild asthma and after bronchial provocation. Am Rev Respir Dis 1989;139:806-817.

8. Saglani S, Malmström K, Pelkonen AS, Malmberg LP, Lindahl H, Kajosaari M, et al. Airway remodeling and inflammation in symptomatic infants with reversible airflow obstruction. Am J Respir Crit Care Med 2005;171:722-727.

9. Blobe GC, Schiemann WP, Lodish HF. Role of transforming growth factor beta in human disease. N Engl J Med 2000;342:1350-1358.

10. Hansen G, McIntire JJ, Yeung VP, Berry G, Thorbecke GJ, Chen L, et al. CD4(+) T helper cells engineered to produce latent TGF-beta1 reverse allergen-induced airway hyperreactivity and inflammation. J Clin Invest 2000;105:61-70.

11. Nakao A. Is TGF-beta1 the key to suppression of human asthma? Trends Immunol 2001;22:115-118.

12. Redington AE, Roche WR, Holgate ST, Howarth PH. Colocalization of immunoreactive transforming growth factor-beta 1 and decorin in bronchial biopsies from asthmatic and normal subjects. J Pathol 1998;186:410-415.

13. Fishman S, Hobbs K, Borish L. Molecular biology of cytokines in allergic diseases and asthma. Immun Allergy Clin North Am 1996;16:613-642.

14. Gordon S, Akopyan G, Garban H, Bonavida B. Transcription factor YY1: structure, function, and therapeutic implications in cancer biology. Oncogene 2006;25:1125-1142.

15. Silverman ES, Palmer LJ, Subramaniam V, Hallock A, Mathew S, Vallone J, et al. Transforming growth factor-beta1 promoter polymorphism C-509T is associated with asthma. Am J Respir Crit Care Med 2004;169:214-219.

16. Guo J, Lin X, Williams MA, Hamid Q, Georas SN. Yin-Yang 1 regulates effector cytokine gene expression and TH2 immune responses. J Allergy Clin Immunol 2008;122:195-201.

17. Yépez SH, Pando RH, Argumedo LS, Paredes MV, Cueto AH, Isibasi A, et al. Therapeutic efficacy of an E. coli strain carrying an ovalbumin allergenic peptide as a fused protein to OMPC in a murine model of allergic airway inflammation. Vaccine 2003;21:566-578.

18. Huerta-Yépez S, Baay-Guzmán GJ, García-Zepeda R, Hernández-Pando R, Vega MI, González-Bonilla C, et al. 2-Methoxyestradiol (2-ME) reduces the airway inflammation and remodeling in an experimental mouse model. Clin Immunol 2008;129:313-324.

19. Seligson DB, Hongo F, Huerta-Yépez S, Mizutani Y, Miki T, Yu H, et al. Expression of X-linked inhibitor of apoptosis protein is a strong predictor of human prostate cancer recurrence. Clin Cancer Res 2007;13:6056-6063.

20. Amishima M, Munakata M, Nasuhara Y, Sato A, Takahashi T, Homma Y, et al. Expression of epidermal growth factor and epidermal growth factor receptor immunoreactivity in the asthmatic human airway. Am J Respir Crit Care Med 1998;157:1907-1912.

21. Vignola AM, Chanez P, Chiappara G, Merendino A, Pace E, Rizzo A, et al. Transforming growth factor-beta expression in mucosal biopsies in asthma and chronic bronchitis. Am J Respir Crit Care Med 1997;156:591-599.

22. Baritaki S, Suzuki E, Umezawa K, Spandidos DA, Berenson J, Daniels TR, et al. Inhibition of Yin Yang 1-dependent repressor activity of DR5 transcription and expression by the novel proteasome inhibitor NPI-0052 contributes to its TRAIL-enhanced apoptosis in cancer cells. J Immunol 2008;180:6199-6210.

23. Huerta-Yépez S, Vega M, Escoto-Chávez SE, Murdock B, Sakai T, Baritaki S, et al. Nitric oxide sensitizes tumor cells to TRAIL-induced apoptosis via inhibition of the DR5 transcription repressor Yin Yang 1. Nitric Oxide 2009;20:39-52.