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ISSN 0188-9796 (Print)

2011, Number 4

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Rev Endocrinol Nutr 2011; 19 (4)

Validation of salivary cortisol measurement in a population of young adults

Lavalle-González FJ, Villarreal-Pérez JZ, González-González G, Montes-Villarreal J, Mancillas-Adame L, Tamez-Pérez HE, Bautista-Medina MA, Valencia-García JE

Language: Spanish
References: 14
Page: 146-148
PDF size: 64.78 Kb.


ABSTRACT

Introduction: Adrenal function is usually assessed by measuring total serum cortisol and urinary free cortisol. The quantification of midnight salivary cortisol has recently been introduced as screening test for hypercortisolism states. Objective: To validate salivary cortisol measurement in a population of healthy young adults, comparing it with serum cortisol determinations. Material and methods: We studied 80 healthy individuals, 46 women and 34 men, with a mean age of 20.6 ± 1.36 years, with body mass index (BMI) › 20 and ‹ 25 kg/m2, and with no history of topical or systemic corticosteroid use in the four weeks prior to sample collection. Saliva and serum samples were collected for cortisol measurement. Central tendency, dispersion measures were calculated together with correlation and concordance coefficients. Results: Height was 1.64 ± 0.08 m, weight 59.74 ± 7.80 kg, BMI 21.95 ± 1.46 kg/m2. Mean serum and salivary cortisol were 15.8 ± 5.1 mg/dL and 5.79 ± 3.08 mg/dL, respectively. The correlation and concordance coefficient was 0.70. Conclusions: Salivary and serum cortisol positively correlate in this healthy, young adult population. Salivary cortisol could be used as an alternative screening test in the assessment of cortisol excess syndromes.


REFERENCES

  1. Arlt W, Stewart PM. Adrenal corticosteroid biosynthesis, metabolism and action. Endocrinol Metab Clin North Am 2005; 34: 293-313, viii.

  2. Williams GH, Dluhy RG. Enfermedades de la corteza supra­rrenal. En: Anthony S. Fauci, Eugene Braunwald, Dennis L. Kasper. Harrison. Principios de Medicina Interna. 16 edición, Editorial McGraw Hill 2005: 2247-2253.

  3. Hucklebridge F, Hussain T, Evans P, Clow A. The diurnal patterns of the adrenal steroids cortisol and DHEA in relation to awakening. Psychoneuroendocrinology 2005; 30: 51-57.

  4. Kudielka BM, Bellingrath S, Hellhammer DH. Further support for higher salivary cortisol levels in “morning” compared to “evening” persons. J Psychosom Res 2007; 62: 595-596.

  5. Gozansky WS, Lynn JS, Laudenslager ML, Kohrt WM. Salivary cortisol determinated by enzyme immunoassay is preferable to serum total cortisol for assessment of dynamic hypothalamic-pituitary-adrenal axis activity. Clin Endocrinol 2005; 63: 336-341.

  6. Trilck M, Flitsch J, Lüdecke DK, Jung R, Petersenn S. Salivary cortisol measurement, a reliable method for the diagnosis of Cushing’s syndrome. Exp Clin Endocrinol Diabetes 2005; 113: 225-230.

  7. Viardot A, Huber P, Puder JJ, Zulewski H, Keller U, Müller B. Reproducibility of nighttime salivary cortisol and its use in the diagnosis of hypercortisolism compared with urinary free cortisol and overnight dexamethasone suppression test. J Clin Endocrinol Metab 2005; 90: 5730-5736.

  8. Poll EM, Kreitschmann-Andermahr I, Langejuergen Y, Stanzel S, Gilsbach JM, Gressner A, Yagmur E. Saliva collection method affects predictability of serum cortisol. Clin Chim Acta 2007; 382: 15-19.

  9. Baid SK, Sinaii N, Wade M, Rubino D, Nieman LK. Radioimmunoassay and tandem mass spectrometry measurement of bedtime salivary cortisol levels: a comparison of assays to establish hypercortisolism. J Clin Endocrinol Metab 2007; 92: 3102-3107.

  10. Gröschl M, Köhler H, Topf HG, Rupprecht T, Rauh M. Evaluation of saliva collection devices for the analysis of steroids, peptides and therapeutic drugs. J Pharm Biomed Anal 2008; 47: 478-486.

  11. Guo T, Taylor RL, Singh RJ, Soldin SJ. Simultaneous determination of 12 steroids by isotope dilution liquid chromatography-photo spray ionization tandem mass spectrometry. Clin Chim Acta 2006; 372: 76-82.

  12. Taylor RL, Grebe SK, Singh RJ. Quantitative, highly sensitive liquid chromatography-tandem mass spectrometry method for detection of synthetic corticosteroids. Clin Chem 2004; 50: 2345-2352.

  13. Gotelli GR, Wall JH, Kabra PM, Marton LJ. Fluorometric liquid-chromatographic determination of serum cortisol. Clin Chem 1981; 27: 441-443.

  14. Gore M, Lester E. Comparison of a fluormetric method and a competitive protein binding assay kit for the determination of plasma hydroxycorticosteroids. Ann Clin Biochem 1975; 12: 160-162.




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