Barroso VG, Colín VA, Morales VM, Osuna ZM, Robledo TG, Ávila LR

Language: Spanish
References: 12
Page: 16-21
PDF size: 319.85 Kb.

ABSTRACT

Background: Successful fertilization in assisted reproductive technology depends mainly on the quality of sperm plasma membrane. In patients who consult for infertility has been found more abnormalities in the plasma membrane translocation of phosphatidylserine as, evidence of early apoptotic events. For this population, “healthy” may be apt to fertilize the female gamete requires techniques to select the free sperm apoptotic damage, such as the “magnetic-activated cell sorting” (MACS).
Objective: To compare sperm selection by swim-up only or in combination with MACS-technique in infertile patients ANMB whose semen sample was used for assisted reproduction technique of high complexity (intracytoplasmic sperm injection ICSI), with the rate of fertilization and embryonic development.
Material and methods: pilot study of 11 couples in whom the semen sample was divided into two groups: (group A: selected by swimup), (group B selected by swim up and MACS). With the population obtained seminal ICSI was performed for each group was evaluated fertilization rate, embryo development and fragmentation.
Results: The fertilization rate was 83.8 vs 93.9% for groups A and B, respectively, for what concerns the number of blastomeres was 2.6 and 2.8 and in the percentage of fragmentation on day three after fertilization of 7.19 vs. 7.88% for groups A and B, respectively, without reaching statistical significance.
Conclusions: It is now clear that sperm selection by MACS decreases the sperm population with early apoptotic damage, however, it is necessary to conduct a clinical trial to determine significant differences in fertilization rate, embryo development, implantation rate and rate of pregnancy.

REFERENCES

1. Kruger TF, Acosta AA, Simmons KF. Predictive value of abnormal sperm morphology in vitro fertilization. Fertil Steril 1988;49:112-117.

2. Barroso G, Valdespin C, Vega E, Kershenovich R, et al. Developmental sperm contributions: fertilization and beyond. Fertil Steril 2009;92(3):835-848.

3. Said TM, Grunewald S, Paasch U, Glander HJ, et al. Advantage of combining magnetic cell separation with sperm preparation techniques. Reprod Biomed Online 2005;10(6):740-746.

4. Said TM, Agarwal A, Grunewald S, Rasch M, et al. Evaluation of sperm recovery following annexin V magnetic-activated cell sorting separation. Reprod Biomed Online 2006;13(3):336-339.

5. Grunewald S, Paasch U, Said TM, Rasch M, et al. Magneticactivated cell sorting before cryopreservation preserves mitochondrial integrity in human spermatozoa. Cell Tissue Bank 2006;7(2):99-104.

6. WHO laboratory manual for the examination and processing of human semen. 5th ed. Switzerland, 2010.

7. Paasch U, Grunewald S, Fitzl G, Glander HJ. Deterioration of plasma membrane is associated with activated caspases in human spermatozoa. J Androl 2003;24(2):246-252.

8. Grunewald S, Reinhardt M, Blumenauer V, Said TM, et al. Increased sperm chromatin decondensation in selected nonapoptotic spermatozoa of patients with male infertility. Fertil Steril 2009;92(2):572-577.

9. Said T, Agarwal A, Grunewald S, Rasch M, et al. Selection of nonapoptotic spermatozoa as a new tool for enhancing assisted reproduction outcomes: an in vitro model. Biol Reprod 2006;74(3):530-535.

10. Said TM, Agarwal A, Zborowski M, Grunewald S, et al. Utility of magnetic cell separation as a molecular sperm preparation technique. J Androl 2008;29(2):134-142.

11. Said TM, Grunewald S, Paasch U, Rasch M, et al. Effects of magnetic-activated cell sorting on sperm motility and cryosurvival rates. Fertil Steril 2005;83(5):1442-1446.

12. Colin A, Gomez-López N, Durán H, Barroso G, Oehninger S. The effect of age on the expression of apoptosis biomarkers in human spermatozoa. Fertil Steril 2011;94:2609-2614.