medigraphic.com
ISSN 0187-5337 (Print)
Instituto Nacional de Perinatología

2011, Number 4

<< Back Next >>

Perinatol Reprod Hum 2011; 25 (4)

Effect of in vitro exposure of human spermatozoa to cadmium (Cdcl2)

Méndez Y, Báez F, Villamediana P

Language: Spanish
References: 34
Page: 198-204
PDF size: 154.75 Kb.


ABSTRACT

The development of anthropogenic activities has allowed environmental dispersion of heavy metals as cadmium; this event is related with decreased male fertility. This study was carried out with the aim of determine the effect of in vitro exposure of human spermatozoa to cadmium. Spermatozoa were incubated in presence of CdCl2, in concentrations of 2 ppm and 4 ppm, and motility, vitality, membrane integrity, sperm capacitation and sperm chromatin integrity was assessed. In vitro exposure to CdCl2 caused a significant effect on moderate motility pattern (p = 0.01) and immobile sperm (p &#$8249; 0.05) whereas it didn’t cause a significant effect on viability, membrane integrity, acrosome reaction, sperm chromatin condensation and sperm chromatin fragmentation. Cadmium caused a deleterious effect on sperm motility; nevertheless, it didn’t cause decrease on viability, membrane or chromatin structure alterations under the concentrations studied in vitro.


REFERENCES

  1. Skakkebaek N, Jorgensen N, Main K; Rajpert-De Meyts E, Leffers H, Andersson A et al. Is human fecundity declining? Int J Androl 2006; 29: 2-11.

  2. Ten J, Mendiola J, Torres-Cantero A, Moreno-Grau J, Moreno-Grau S, Roca M et al. Occupational and lifestyle exposures and male infertility: A mini review. The Open Reproductive Science Journal 2008; 1: 16-21.

  3. OSHO, Occupational safety and health office. Exposición ocupacional a cadmio; regla final. Departamento del Trabajo y los Recursos Humanos de Puerto Rico. 1992.

  4. Ramírez A. Toxicología del cadmio. Conceptos actuales para evaluar exposición ambiental u ocupacional con indicadores biológicos. An Fac Med, Univ Mayor San Marcos 2002; 63: 51-64.

  5. OSHA, Occupational Safety And Health Administration. Cadmium. U.S Department of Labor. OSHA 3136-06R 2004.

  6. Benoff S, Jacob A, Hurley, I. Male infertility and environmental exposure to lead and cadmium. Hum Reprod Upd 2000; 6: 107-21.

  7. Monsefi M, Alaee S, Moradshahi A, Rohani L. Cadmium-induced male infertility in male mice. Environ Toxicol 2009; 25: 94-102.

  8. Chung N, Cheng Y. Is cadmium chloride-induced inter-sertoli tight junction permeability barrier disruption a suitable in vitro model to study the events of junction disassembly during spermatogenesis in the rat testis? Endocrinology 2001; 142: 1878-88.

  9. Benoff S, Centola G, Millan C, Napolitano B, Maumar J, Hurley I. Increased seminal plasma lead levels adversely affect the fertility potential of sperm in IVF. Hum Reprod 2003; 18: 374-83.

  10. Leoni G, Bogliolo L, Deiana G, Berlinguer F, Rosati I, Pintus P et al. Influence of Cadmium exposure on in vitro ovine gamete dysfunction. Reprod Toxicol 2002; 16: 371-7.

  11. El-Shahat A, Gabr A, Meki A, Mehana E. Altered testicular morphology and oxidative stress induced by cadmium in experimental rats and protective effect of simultaneous green tea extract. Int J Morphol 2009; 27: 757-64.

  12. Wong C, Mruk D, Lui W, Cheng C. Regulation of blood-testis barrier dynamics: an in vivo study. J Cell Sci 2004; 117: 783-98.

  13. Younis A, Zuelke K, Harper K, Oliveira M, Brackett B. In vitro fertilization of goat oocytes. Biol Reprod 1991; 44: 1177-82.

  14. WHO, World Health Organization. WHO Laboratory manual for the examination and processing of human semen. 5th ed. Switzerland: World Health Organization; 2010.

  15. Giraldo N, Correa J, Vásquez N. Evaluación del efecto de la refrigeración sobre la calidad del semen equino. Revista CES 2006; 2: 8-16.

  16. Valeris R, Villamediana P, Quintero A, Parra O, Sandoval J, Ghebrehiwet B. Expression and distribution of the complement receptor gC1qR in bovine sperm. Revista Científica, FCV-LUZ 2008; 18: 22-7.

  17. Sousa A, Tavares R, Velez J, Figueiredo H, Almeida V, Almeida-Santos T et al. Dual use of Diff-Quick-like stains for the simultaneous evaluation of human sperm morphology and chromatin status. Hum Reprod 2009; 24: 28-36.

  18. Fernández J, Muriel L, Rivero M, Goyanes V, Vázquez R, Alvarez J. The sperm chromatin dispersion test: A simple method for the determination of sperm DNA fragmentation. J Androl 2003; 24: 59-66.

  19. SAS, Statistical Analysis System Institute. Sas/stat user´s guide, 8.2 edition. Cary, NC. USA. 2001.

  20. Arabi M, Mohammadpour A. Adverse effects of cadmium on bull spermatozoa. Vet Res Commun 2006; 30: 943-51.

  21. Oliveira H, Spanò M, Santos C, Pereira M. Adverse effects of cadmium exposure on mouse sperm. Reprod Toxicol 2009; 28: 550-5.

  22. Schlingmann K, Michaut M, McElwee J, Wolff C, Travis A, Turner R. Calmodulin and CaMKII in the sperm principal piece: Evidence for a motility-related calcium/calmodulin pathway. J Androl 2007; 28: 706-16.

  23. Lindemann C, Gardner T, Westbrook E, Kanous K. The calcium-induced curvature reversal of rat sperm is potentiated by cAMP and is inhibited by anti-calmodulin. Cell Motil Cytoskel 1991; 20: 316-24.

  24. Kanous K, Casey C, Lindemann C. Inhibition of microtubule sliding by Ni2+ and Cd2+: evidence for a differential response of certains microtubule pairs within the bovine sperm axoneme. Cell Motil Cytoskel 1993; 26: 66-76.

  25. Lee N, Cheng C. Adaptors, junction dynamics and spermatogenesis. Biol Reprod 2004; 71: 392-404.

  26. Wong C, Mruk D, Lui W, Cheng C. Regulation of blood-testis barrier dynamics: an in vivo study. J Cell Sci 2004; 117: 783-98.

  27. Braydich-Stolle L, Hussain S, Schlager J, Hofmann M. In vitro cytotoxicity of nanoparticles in mammalian germline of stem cells. Toxicol Sci 2005; 88: 412-9.

  28. Ingersoll R, Wasserman R. Vitamin D-induced calcium-binding protein. J Biol Chem 1971; 246: 2808-14.

  29. Brandelli A, Miranda P, Tezon J. Voltage-dependent calcium channels and Gi regulatory protein mediate the human sperm acrosomal exocytosis induced by N-acetylglucosaminyl/mannosyl neoglycoproteins. J Androl 1996; 17: 552-529.

  30. Benoff S, Hurley I, Mandel F, Cooper G, Hershlag A. Induction of the human sperm acrosome reaction with mannose-containing neoglycoprotein ligands. Mol Hum Reprod 1997; 3: 827-837.

  31. Amara S, Abdelmelek A, Garrel C, Guiraud P, Douki T, Ravanat J et al. Preventive effect of zinc against cadmium-induced oxidative stress in the rat testis. J Reprod Develop 2008; 54: 129-134.

  32. Sergerie M, Ouhilal S, Bissonnette F, Brodeur J, Bleau G. Lack of association between smoking and DNA fragmentation in the spermatozoa of normal men. Hum Reprod 2000; 15: 1314-1321.

  33. Sepaniak S, Forges T, Gerard H, Foliguet B, Bene M, Monnier-Barabarino P. The influence of cigarette smoking on human sperm quality and DNA fragmentation. Toxicology 2006; 223: 54-60.

  34. Björndahl L, Kvist U. Human sperm chromatin stabilization: A proposed model including zinc bridges. Mol Hum Reprod 2010; 16: 23-29.




2020     |     www.medigraphic.com