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Instituto Nacional de Salud Pública

2011, Number 6

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salud publica mex 2011; 53 (6)

CCR5-Δ32 allele involvement in the clinical evolution of HIV1+ patients in Yucatán, Mexico

Valadez-González N, González-Martínez P, Lara-Perera D, Vera-Gamboa L, Góngora-Biachi R

Language: Spanish
References: 34
Page: 463-468
PDF size: 110.45 Kb.


ABSTRACT

Objective. CCR5-Δ32 allele frequency needs to be identified in HIV+ patients and exposed seronegative individuals in Yucatan, Mexico, to understand this mutation’s relationship to infection and disease progression. Material and Methods. A total of 355 samples were analyzed: 62 from HIV+ patients, 51 from exposed seronegative individuals and 242 from general population. Infected patients were subdivided into a) normal progressors n= 49; b) slow progressors n=10, and c) non-progressors n=3. Results. Genotype wt/Δ32 was identified in 17.7% of HIV+, 13.7% of exposed seronegative individuals and 6.2% of general population. Genotype Δ32/Δ32 was identified in 3.9% of exposed seronegative individuals. In infected patients, wt/Δ32 was identified in 10.2% of normal progressors, 30% of slow progressors and 100% of nonprogressors. Conclusion. Genotype wt/Δ32 was observed in all non-progressing HIV+ patients, supporting its role in this group’s disease development and clinical evolution.


REFERENCES

  1. Dagleish AG, Beverley PCL, Clapham PR, Crawford DH, Greaves MF, Weiss RA. The CD4 antigen is an essential component of the receptor for the AIDS retrovirus. Nature 1984;312:763-767.

  2. Feng Y, Broder CC, Kennedy PE, Berger EA. HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science 1996;272:872-877.

  3. Dragic T, Litwin V, Allaway GP, Martin SR, Huang Y, Nagashima KA, et al. HIV-1 entry into CD4+ cells is mediated by the chemokine receptor CCCKR-5. Nature 1996;381:667-673.

  4. Cocchi F, DeVico AL, Garzino-Demo A, Arya SK, Gallo RC, Lusso P. Identification of RANTES, MIP-1 alpha and MIP-1 beta as the major HIVsuppressive factors produced by CD8+ cells. Science 1995;270:1811-1815.

  5. Choe H, Farzan M, Sun Y, Sullivan N, Rollins B, Ponath PD, et al. The betachemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates. Cell 1996;85:1135-1148.

  6. Liu R, Paxton WA, Choe S. Homozygous defect in HIV-1 coreceptor accounts for resistance of some multiply –exposed individuals to HIV-1 infection. Cell 1996;86:367-377.

  7. Samson M, Libert F, Doranz BJ, Rucker J, Liesnard C, Farber CM, et al. Resistance to HIV-1 infection in Caucasian individuals bearing mutant alleles of the CCR-5 chemokine receptor gene. Nature 1996;382:722-725.

  8. Paxton WA, Martin SR, Tse D, O’Brien TR, Skurnick J, VanDevanter NL, et al. Relative resistance to HIV infection of CD4 lymphocytes from persons who remain uninfected despite multiple high risk sexual exposures. Nat Med 1996;2:412-417.

  9. Connor RI, Paxton W, Sheridan KE, Koup R. Macrophages and CD4+ T Lymphocytes from two multiply exposed uninfected individuals resist infection with primary non-syncytium-inducing isolates of human immunodeficiency Virus Type 1. J Virol 1996;70:8758-8764.

  10. Xiao L, Weiss SH, Qari SH, Rudolph D, Zhao C, Denny TN, et al. Partial resistance to infection by R5X4 primary HIV type 1 isolates in an exposed-uninfected individual homozygous for CCR5 32-base pair deletion. AIDS Res Hum Retroviruses 1999;15:1201-1208.

  11. Bradbury J. HIV-1-resistant individuals may lack HIV-1 coreceptor. Lancet 1996;348:463.

  12. Eugen-Olsen J, Iversen AKN, Garred P, Koppelhus U, Pedersen C, Benfield TL, et al. Heterozygosity for a deletion in the CKR-5 gene leads to prolonged AIDS-free survival and slower CD4 T cell decline in a cohort of HIV-seropositive individuals. AIDS 1997;11:305-310.

  13. Cohen OJ, Paolucci S, Bende SM, Daucher M, Moriuchi H, Moriuchi M, et al. CXCR4 and CCR5 genetic polymorphisms in long-term nonprogressive human immunodeficiency virus infection: lack of association with mutations other than CCR5- Δ32. J Virol 1998;72:6215-6217.

  14. Eskild A, Jonassen TO, Heger B, Samuelsen SO, Grinde B. The estimated impact of the CCR-5 Δ 32 gene deletion on HIV disease progression varies with study design. AIDS 1998;12:2271-2274.

  15. Philpott S, Burger H, Charbonneau T, Grimson R, Vermund SH, Visosky A, et al. CCR5 Genotype and Resistance to Vertical Transmission of HIV-1. J AIDS 1999;21:189-193.

  16. González-Díaz E, Villasís KA, Ruiz-Palacios GM, Soto-Ramírez LE. Genotipo del receptor CCR5 en individuos mexicanos infectados por VIH con progresión normal, lentos progresores y expuestos pero no infectados. En: Calva Mercado JJ (ed). Enfermedades Infecciosas y Microbiología. XXIII Congreso Anual de la Asociación Mexicana de Infectología y Microbiología Clínica. V Congreso Nacional de Antimicrobianos y Quimioterapia 1998;18:S29.

  17. Quintal-Ortiz I. Identificación de los polimorfismos m555 y m303 en el gen que codifica para el receptor CCR5 en una población de la etnia Maya y Mestiza del Estado de Yucatán (tesis). Mérida (YUC): Universidad Autónoma de Yucatán, 2007.

  18. Góngora-Biachi RA, González-Martínez P, Puerto-Manzano F, Franco- Monsreal J. Transmisión heterosexual del virus de inmunodeficiencia humana tipo 1 en un grupo de parejas residentes de la Península de Yucatán. Rev Invest Clin 1991;43:128-132.

  19. Góngora-Biachi RA, González-Martínez P. Anticuerpos contra el virus de la inmunodeficiencia humana (VIH) en una población de prostitutas de Mérida, Yucatán, México. Rev Invest Clin 1989;39:305-306.

  20. Góngora-Biachi RA, González-Martínez P, Castro-Sansores C, Pavia- Ruz N, Rudolph DL, Lal RB. et al. Human T lymphotropic virus type II (HTLV-II) infection among female prostitutes in Yucatan, Mexico. Am J Med Sci 1993;306:207-211.

  21. Góngora-Biachi RA, Pavía-Ruz N, González-Martínez P, Puerto- Manzano F. Female prostitution and HIV infection in Yucatán, México. Fourth RCM International AIDS Symposium, San Juan Puerto Rico. November 3-4 1994:56.

  22. Góngora-Biachi RA, González-Martínez P, Reyes-Pinto A, Lara-Perera D, López-Peraza A, Medina-Escobedo G. Prevalencia de anticuerpos contra el virus de la inmunodeficiencia humana (Ac-VIH) y su expresión clínica en un grupo de homosexuales del sexo masculino en Mérida, Yucatán. Salud Publica Mex 1987;29:474-480.

  23. Góngora-Biachi RA, Arcila-Herrera H, González-Martínez P, Franco-Monsreal J, Puerto-Manzano F, Martinez-Reynoso A, et al. Anticuerpos contra el Virus de la Inmunodeficiencia Humana en una población homosexual masculina. Salud Publica Mex 1990;32:20-25.

  24. Froebel KS, Doherty KV, Whitelaw JA, Hague RA, Mok JY, Bird AG. Increased expression of the CD45RO (memory) antigen on T cells in HIVinfected children. AIDS 1991;5:97-99.

  25. Greenough TC, Brettler DB, Kirchhoff F, Alexander L, Desrosiers RC, O’Brien SJ, et al. Long-term nonprogressive infection with human immunodeficiency virus Type 1 in a hemophilia cohort. J Infect Dis 1999;180:1790-1802.

  26. Higuchi R. Simple and rapid preparation of samples for PCR. En: Erlich HA, ed. PCR Technology. Nueva York: Stockton Press, 1989:31-38.

  27. Zimmerman PA, Bucklerwhite A, Alkhatib G, Spalding T, Kubofcik J, Combadiere C. Inherited resistance to HIV-1 conferred by an inactivating mutation in CC chemokine receptor 5-studies in populations with contrasting clinical phenotypes, defined racial background, and quantified risk. Mol Med 1997;3:23-36.

  28. Trecarichi EM, Tumbarello M, de Gaetano-Donati K, Tamburrini E, Cauda R, Brahe C, et al. Partial protective effect of CCR5-Delta 32 heterozygosity in a cohort of heterosexual Italian HIV-1 exposed uninfected individuals. AIDS Res Ther 2006;3:22-25.

  29. Rugeles-López MT, Díaz FJ, Vega JA, Solano F, Nagles J, Bedoya G, et al. Frecuencia de mutaciones en el correceptor CCR5 del virus de la Inmunodeficiencia Humana (VIH) en diferentes grupos en Medellín. Infectio 2001;5:87-95.

  30. Dean M, Carrington M, Winkler C, Huttley GA, Smith MW, Allikmets R, et al. Genetic restriction of HIV-1 infection and progression to AIDS by a deletion allele of the CKR5 structural gene. Science 1996;273:1856-1862.

  31. Buseyne F, Janvier G, Teglas JP, Ivanoff S, Burgard M, Bui E, et al. Impact of heterozygosity for the chemokine receptor CCR5 32-bp-deleted allele on plasma virus load and CD4 T lymphocytes in perinatally human immunodeficiency virus-infected children at 8 years of age. J Infect Dis 1998;178:1019-1023.

  32. Carrington M, Nelson G, Martin MP, Kissner T, Vlahov D, Goedert JJ. HLA and HIV-1: Heterozygote advantage and B*35-Cw*04 disadvantage. Science 1999;283:1748-1752.

  33. Carrington M, O’Brien SJ. The influence of HLA genotype on AIDS. Annu Rev Med 2003;54:535-551.

  34. Salas-Alanis JC, Mellerio JE, Ashton GHS, McGrath JA. Frequency of the CCR5 gene 32-base pair deletion in Hispanic Mexicans. Exp Dermatol 1999;24:127-129.




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