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Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado

2010, Number 4

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Rev Esp Med Quir 2010; 15 (4)

Insulin, leptin and insulin-resistance degree in school children with and without obesity

González YMGE, Madero FCMA, Martínez OVA, Serrano GLB

Language: Spanish
References: 59
Page: 196-203
PDF size: 91.37 Kb.


ABSTRACT

Background: Leptin is a hormone of the adipose tissue that reduces the appetite and the corporal fat; the insulin is produced in the pancreatic cells beta, the function is to transport the glucose through the fatty, muscular and hepatic tissues. The excess of fatty tissue is associated to hyperleptinemia, hyperinsulinemia, cardiovascular risks, diabetes and hypertension. There is a relation between infancy obesity and the metabolic alterations that are mentioned above.
Objective: To determine serum insulin and leptin levels and insulin resistance among primary school children with and without obesity.
Participants and method: A transversal study was performed with children from an elementary school (six to nine years old). From the 282 scholars checked only 80 were randomly selected from first and fourth body mass index (BMI) quartile. Data about gender, weight, height, waist circumference, blood pressure, BMI, percentage of BMI, and Z score were recorded. Platelet, leucocytes, hematocrit, hemoglobin, triglycerides, cholesterol, glucose, HOMA (resistance to insulin), insulin and leptin were also measured.
Results: 37 and 43 children were selected of first and fourth quartile, respectively. The levels (mean ± SD) of leptin were: 3.34 ± 2.74 and 26.14 ± 18.17 (p ‹ 0.001), insulin 10.74 ± 15.31 and 24.37 ± 28.57 (p = 0.009). Leptin quartiles showed significant differences in weight (p ‹ 0.001), height (p ‹ 0.001), waist circumference (p ‹ 0.001), BMI (p ‹ 0.001), BMI percentile (p ‹ 0.001), Z score (p ‹ 0.001), triglycerides (p ‹ 0.001), insulin (p ‹ 0.001), and HOMA (p ‹ 0.001).
Conclusion: There are differences in metabolic parameters and leptin concentration among groups, leptin could be an early marker of infancy obesity.


REFERENCES

  1. Martorell R. La diabetes y los mexicanos: ¿Por qué están vinculados? Prev Chronic Dis 2005;2(1):1-6.

  2. Instituto Nacional de Estadísticas Geográficas e Informática. (INEGI). Prevalencia de enfermedad crónicas no trasmisibles y factores de riesgo cardiovascular. México: Censo Geografía e Informática, 2000.

  3. Secretaría de Salud. Norma Oficial Mexicana NOM-174-SSA1-1998, Para el manejo integral de la obesidad. Acuerdo secretarial 141. Publicada en el Diario Oficial de la Federación el 29 de diciembre de 1997. Fecha de publicación 12 de abril de 2000.

  4. Zimmet P, Thomas CR. Genotype, obesity and cardiovascular disease – has technical and social advancement outstripped evolution?. J Intern Med 2003;254:114-112.

  5. James PT, Rigby N, Leach R. The obesity epidemic, metabolic syndrome and future prevention strategies. Eur J Cardiovasc Prev Rehabil 2004; 11(Pt 1):3-8.

  6. Fariñas RL, Meléndez MM, Martínez Z, Travieso Y, et al. Control de la alimentación y leptina. Rev Cub Invest Biomed 2005;24(Pt 1):47.

  7. Simón E, Barrio AS. Leptina y obesidad. Ana 2007; 25 (1 Supl 6): 1-17.

  8. Almaraz RL, Díaz GJ, Perera R. Leptina: conocimientos actuales e implicaciones clínicas. Can Ped 2000;24(Supl 3):159-156.

  9. Christos S, Mantzoros MD. The role of leptin in human obesity and disease: a review of current evidence. Ann Intern Med 1999;130:671-610.

  10. Hung YJ, Chu NF, Wang SC, Hsieh CH, et al. Correlation of plasma leptin and adiponectin with insulin sensitivity and beta-cell function in children-the Taipei Children Heart Study. Intern J Clin Pract 2006;60(12):1582-1587.

  11. Abbatecola MA, Ferrucci L, Grella R, Bandinelli S, et al. Diverse effect of inflammatory markers on insulin resistance and insulin-resistance syndrome in the elderly. J Am Geriatric Soc 2004;52:399-404.

  12. Martos-Moreno GA, Barrios V, Argente J. Normative data for adiponectin, resistin, interleukin 6, and leptin/receptor ratio in a healthy Spanish pediatric population: relationship with sex steroids. Eur J Endocrinol 2006;155(Supl 3):429-426.

  13. Wikipedia Fundation, Inc. Leptin [serial on line] 2006 [cited 2006 Dic 12]; [4 screen]. Available from: http://en.wikipedia.org/wiki/Leptin

  14. Friedman JM, Halaas JL. Leptin and the regulation of body weight in mammals. Natural Publisher 1998;395:763-767.

  15. Kolaczynski JW, Considine RV, Ohannesian J, Marco Ch, et al. Responses of leptin to short-term fasting and refeeding in humans. A link with ketogenesis but not ketones themselves. Diabetes 1996;45:1511-1515.

  16. Zimmet P, Hodge A, Nicolson M, Staten M, et al. Serum leptin concentration, obesity, and insulin resistance in Western Samoans: cross sectional study. BMJ 1996;313:965-964.

  17. Russell CD, Peterson RN, Rao SP, Ricci MR, et al. Leptin expression in adipose tissue from obese humans: depotspecific regulation by insulin and dexamethasone. Am J Physiol Society 1998;275(38):507-509.

  18. Banerjee I, Trueman JA, Hall CM, Price DA, et al. Phenotypic variation in constitutional delay of growth and puberty: relationship to specific leptin and leptin receptor gene polymorphisms. Eur J Endocrinol 2006;155(Supl 1):121-126.

  19. Shalitin S, Phillip M. Role of obesity and leptin in the pubertal process and pubertal growth – a review. Int J Obes 2003;27:869-865.

  20. Viner R, Booy R. Epidemiology of health and illness. Clin Rev 2005;330(3 Supl 12):411-414.

  21. Valle M, Martos R, Gascón F, Cañete R, et al. Low-grade systemic inflammation, hypoadiponectinemia and a high concentration of leptin are present in very young obese children, and correlate with metabolic syndrome. Diabetes Metab 2005;31:55-58.

  22. Bouret GS, Simerly BR. Minireview: Leptin and development of hypothalamic feeding circuits. J Endocrinol 2004;145(Supl 6):2621-2626.

  23. Moreno LA, Pineda I, Rodriguez G, Fleta J, et al. Leptin and metabolic syndrome in obese non-obese children. Horm Metab Res 2002;34:394-396.

  24. Nain-Feng C, Muh-Han S, Der-Min W, Chen-Ju L. Relationship between plasma adiponectin levels and metabolic risk profiles in Taiwanese children. Obes Res 2005;13:2014-2017.

  25. Kelesidis T, Mantzoros CS. The emerging role of leptin in humans. Pediatr Endocrinol Rev 2006;3(Pt 3):239-248.

  26. Diagnsotic Systems Laboratories, Inc. Human Insulin Elisa Cat # Active DSL-10-1600 -Rev - January 12, 2007.

  27. Linco Research Human Leptin Elisa kit Cat # EZHL-80SK – Rev. Mar 15 06.

  28. Segal KR, Landt M, Klein S. Relationship between insulin sensitivity and plasma leptin concentration in lean and obesity men. Pediatr Diabetes 1996;45:988-984.

  29. Children’s Nutrition Research Center of Medicine. BMI Graph Page [serial on line] 2007 CDC Growth Chart [cited 2007 feb 15]:[3 screens]. Available from: URL:http://www.kidsnutrition.org/bodycomp/bmiz2.htlm

  30. BioSystems S.A. Concepto. Colesterol. Enzimático-espectrofotométrica colesterol oxidasa/peroxidasa.

  31. BioSystems S.A. Concepto. Triglicéridos. Enzimáticaespectrofotométrica glicerol fosfato oxidasa/peroxidasa.

  32. BioSystems S.A. Concepto. Glucosa. Enzimática-espectrofotométrica glucosa oxidasa/peroxidasa.

  33. Bioquímica clínica Spectrum® [computer program]. Versión 4.8 Abbott system; 1995.

  34. Abbott Spectrum®. System Reagents. Diagnostic Division, 1921 Hurd, P.O. Box. 1995;p:49,65,105.

  35. Manual Celdyn 3300. Control de Calidad. Abbott; 1995;2:7.

  36. Manual Celdyn 3500. Principios de Operación. Abbott; 1995;1:3.

  37. Manual Plate-Reader das.srl. CE. Versión A1. Palombara Sabina Roma Italia: APPAR; 2000.

  38. Reinehr T, Kratzsch J, Kiess W, Andler W. Circulation soluble leptin receptor, leptin, and insulin resistance before and after weight loss in obese children. Int J Obes (Lond) 2005;29(Pt 10):1230-1235.

  39. Lönnerdal B, Havel PJ. Serum leptin concentrations in infants. Am J Clin Nutr 2000;72:484-489.

  40. Dencker M, Thorsson O, Karlsson MK, Linden C, et al. Leptin is closely related to body fat in prepubertal children age 8-11 years. Acta Pediatr 2006;95(Supl 8):975-979.

  41. Tresaco B, Bueno G, Pineda I, Moreno LA, et al. Homeostatic model assessment (HOMA) index cut-off values to identify the metabolic syndrome in children. J Physiol Biochem 2005;61(Supl 2):381-388.

  42. Whipple T, Sharkey N, Demers L, Williams N. Leptin and the skeleton. Clin Endocrinol 2002;57:701-711.

  43. Astrup A, Hansen DL, Lundsgaard C, Toubro S. Sibutramine and energy balance. Int J Obes 1998;22(Supl 1):30-36.

  44. Sato T, Laviano A, Meguid MM, Chen C, et al. Involvement of plasma leptin, insulin and free tryptophan in cytokineinduced anorexia. Clin Nutr 2003;22(Pt 2):139-146.

  45. Reinehr T, Kratzsch J, Kiess W, Andler W. Circulation soluble leptin receptor, leptin, and insulin resistance before and after weight loss in obese children. Int J Obes (Lond) 2005;29(Pt 10):1230-1235.

  46. López A J, González GL. Enfermedades asociadas a la obesidad. Endocrinol Nutr 2001;9(Supl 2):77-79.

  47. Porte DJr, Baskin DG, Schwartz MW. Leptin and insulin action in the central nervous system. Nutr Rev 2002; 60(Pt 2):S20-9; discussion S68-84, 85-87.

  48. Lodish H, Berk A, Zpurski LS, Matsudaira P, et al. Biología celular y molecular. 4ª ed. España: Editorial Médica Panamericana, 2003;p:852-899.

  49. Hulver MW. Dohm GL. The molecular mechanism linking muscle fat accumulation to insulin resistance. Proc Nutr Soc 2004;62(Supl 2):375-380.

  50. Shinozaki K, Kashiwagi A, Okamura T. Molecular mechanisms of impaired endothelial function associated with insulin resistance. J Curr Drug Targets Cardiovasc Haematol Disord 2004;4(Supl 1):1-11.

  51. Wikipedia Foundation, Inc. Insulin. [serial on line] 2006 [cited 2006 Dec 22]; [25 screen]. Available from: URL:http://en.wikipedia.org/wiki/insulin

  52. Havel JP. Role of adipose tissue in body-weight regulation: mechanism regulating leptin production and energy balance. Proceed Nutr Soc 2000;59:359-371.

  53. Moran O, Phillip M. Leptin: obesity, diabetes and other peripheral effect – a review. Pediatric Diabetes 2003;4:101-109.

  54. Holm JC, Gamborg M, Kaas-Ibsen K, Gammeltoft S, et al. Time course and determinants of leptin decline during weight loss in obese boys and girls. Int J Pediatr Obes 2007;2(1):2-10.

  55. Dubern B, Girardet JP, Tounian P. Insulin resistance and ferritin as major determinants of abnormal serum aminotransferase in severely obese children. Int J Pediatr Obes 2006;1(2):77-82.

  56. Gunturu SD, Ten S. Complications of obesity in childhood. Pediatr Ann 2007;36(2):96-101.

  57. Kelly AS, Steinberger J, Kaiser DR, Olson TP, et al. Oxidative stress and adverse adipokine profile characterize the metabolic syndrome in children. J Cardiometab Syndr 2006;1(4):248-252.

  58. Pacifico L, Celestre M, Anania C, Paolantonio P, et al. MRI and ultrasound for hepatic fat quantification: relationships to clinical and metabolic characteristics of pediatric nonalcoholic fatty liver disease. Acta Pediatr 2007;96(4):542-547.

  59. MatsuyamaT, Shoji K, Watanabe H, Shimizu M, et al. Effects of diacylglycerol oil on adiposity in obese children: initial communication. J Pediatr Endocrinol Metab 2006;19:795-804.




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