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2011, Number 3

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Cir Cir 2011; 79 (3)

Enzymatic detergent in rat peritoneum. A comparative study with physiological solution

Arteaga-Torres R, Escartín-Chávez M, Gutiérrez-Samperio C, Ruiz-Acosta JM, Alonso-Gallegos M, Lerma-Alvarado R

Language: Spanish
References: 34
Page: 250-256
PDF size: 345.72 Kb.


ABSTRACT

Background: Peritoneal washing out with physiological solution added with different substances is useful in peritoneal infections, but the effect of enzymatic detergents such as quaternary didecyl-dimethyl ammonium compounds (DDAC) used in the sterilization of surgical material is unknown. We undertook this study to determine histological changes (inflammation, fibrosis and new vessel formation) in peritoneum of Wistar rats after the application of physiological solution or DDAC.
Methods: The minimum inhibitory concentration (MIC) of DDAC for E. coli (512 µg/ml) and E. faecalis (128 µg/ml) was determined. Sixty three Wistar rats weighing 200 ± 20 g were studied. They were divided into three groups: control—7 rats were instilled with 3 ml of physiological solution in peritoneal cavity; groups 1 and 2 were instilled with 3 ml of MIC for E. coli and E. faecalis, respectively. These groups were divided into four subgroups of seven animals. In every rat, 1 cm² of peritoneum was obtained at 2, 7, 14, and 21 days for histological study with hematoxylin-eosin. Ten fields were evaluated. Data obtained were analyzed with the Mann-Whitney test.
Results: There were no significant differences in inflammation, fibrosis and new vessel formation with the physiological solution vs. DDAC at 2, 7, 14, and 21 days (p ›0.05), except for inflammation at 2 days in group 2 (p = 0.026), which remitted.
Conclusions: There was no significant difference in changes in rat peritoneum after physiological solution or DDAC application.


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