Rivera TJA, Castillo VLV, Sánchez HJA

Language: Spanish
References: 16
Page: 60-62
PDF size: 173.65 Kb.

ABSTRACT

introduction. Cell cultures are widely used in both biomedical and biotechnological research centers and industry, as well as for diagnostic tests in hospitals. Contamination of cell cultures with microbial organisms as well as with virus or other eukaryotic cell lines are a major problem in cell culture related research.
objective. Mycoplasmas detection in cell cultures came from biomedical laboratories.
material and methods. The cell cultures screened for mycoplasmas by using microbiological culture and PCR. Cell cultures were grown in the absence of antibiotics for 3-4 days. Detection of mycoplasmas was based on alterations in the pH of the broth in the absence of turbidity, production of colonies with the aspect of fried eggs. Comparison between microbiological and PCR were made using Student T test.
results. Mycoplasmas were detected by culture in 9/20 (45%) of the cell culture samples and PCR revealed the presence of target DNA in 10/20 (50%) samples. Mycoplasmas detection between the microbiological culture and PCR show no significant differences (P›0.05).
conclusion. Mycoplasmas detection in cell cultures must be strengthened with another technique to validate the results.

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