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Organo Oficial de la Sociedad Mexicana de Urología

2008, Number 4

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Rev Mex Urol 2008; 68 (4)

Preliminary study of Acid-Base behavior in spermatic subpopulations with gasometry

Gómez-Arzapalo VE, Herrera-Ávalos I, García de la Paz M, Uribe-Uribe MI, Villacorta-Argaez A

Language: Spanish
References: 24
Page: 215-219
PDF size: 145.82 Kb.


ABSTRACT

Introduction: Provided that O2 saturation is carried out in an appropriate environment, wet mount seminal gasometry on fertile men presents an O2 hypersaturation –higher than arterial blood gases– with a pH that fluctuates between 7.2 and 7.6. Saturation, O2 concentration in seminal plasma and especially excess base saturation (EBS) inversion are all lower in men presenting with fertility problems.
Objective: To determine pH and gas behaviour in spermatic populations in couples presenting with infertility.
Materials and Methods (Design): The possible causes of infertility were analyzed in couples presenting with primary and secondary infertility during their reproductive stage. Twenty-four semen samples from 27- to 45-year old men were processed. Semen sample studies were carried out using the ABL.330 Radiometer 1990 Gas Analyzer.
Results: In the gasometry, both O2 concentration and saturation in the semen plasma samples were carried out in a hyperbaric environment. Oxygen concentration and saturation were compared with those in arterial and venous hematic fluids and the results tended to show the concentration and saturation in normal semen samples with confirmed fertility to be higher than those in the hematic fluids. On the other hand, O2 concentration and saturation in semen samples with subfertility were lower. In the base elements, especially EBS, with a correlation coefficient between EBS and morphologically abnormal cells of – 0.489, these negative figures show an obvious increase in teratogenesis.
Conclusions: Oxygen concentration as well as saturation figures are higher in semen plasma than in arterial concentration. Semen pH fluctuates from 7.2 to 7.6. Hypoxia values were 100 mm of Hg in PO2 or lower. EBS modifications, which have morphological repercussions affecting cell dynamics, are shown.


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