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Organo Oficial de la Sociedad Mexicana de Urología

2008, Number 1

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Rev Mex Urol 2008; 68 (1)

In non-preserving surgery: the hyperbaric chamber accelerates the time of recovery. Histological and functional evidence with nerves interposed in an experimental model

Figueroa-Granados V, Raquel EO, Venegas AAS, Sánchez CE, Hernández-Pando R

Language: English
References: 28
Page: 36-43
PDF size: 379.84 Kb.


ABSTRACT

The effect of hyperbaric oxygen (HBO2) therapy on peripheral nerve after its transection and repair, using microsurgical technique, was studied using the rat sciatic nerve model. Following repair, 50 animals were randomly assigned to four groups: 1) No HBO2, sectioned and repaired (n = 10) killed at 7 weeks. 2) HBO2, sectioned and repaired (n = 10) killed at 7 weeks. 3) No HBO2, sectioned and repaired (n = 10) killed at 14 weeks. 4) HBO2, sectioned and repaired (n = 10) killed at 14 weeks. Nerve recovery was assessed by neurophysiological studies (EMG and motor latency) comparing the response before nerve section and after repair, at base line, and after 7 or 14 weeks in all groups. Electromyography was performed comparing HBO2 treated rats and no treated controls after 7 and 14 weeks. Foot-ankle angle response (dorsiflexion) was assessed and histopathology with automated morphometry (axon number, myelin area, blood vessels number) was performed after 7 or 14 weeks. At 7 weeks, motor latency increased significantly in the control non-treated group, whereas amplitude was higher in the HBO2 treated group together with better foot-ankle angle response, higher axons and blood vessels number, and an increase in myelin surface area. After 14 weeks, electromyography showed moderate denervation (90%) and a better foot-ankle angle response in groups treated with HBO2. These results suggest that functional recovery in transected peripheral nerves may be improved by hyperbaric oxygenation following microsurgical repair its aplication can be extended to the radical prostatectomy with sural nerve interposition.


REFERENCES

  1. Panayotis, KT, Okajima S, Terzis JK. Ultrastructure and cellular biology of nerve regeneration. J Recons Microsurg. 1998;14:423-436.

  2. Hampson N. Hyperbaric Oxygen Therapy: A Committe Report. Undersea Hyperbaric Medical Society. 1999;1(5):19.

  3. Yu WY, Shimm SS. The effect of hyperbaric oxygen on nerve regeneration, an experimental study in the femoral nerve of rabbits. In: Proceedings on the 5th International Congress on hyperbaric medicine. Burnaby, B.C., Canada; Simon Fraser University. 1974: 473-478.

  4. Gignoux M, Firica A, Ray A. Effects of ischemia and hyperbaric oxygen on the neuromuscular excitability of the dog paw applications preliminary to reimplantation of the limb. Lyon Chir. 1970; 66:167-170.

  5. Takahashi M, Hirose N, Takeuchi H, Minamiguchi T, Kyo S. Clinical and electrophysiological evaluation of hyperbaric oxygenation in SMON. Nippon Rinsho. 1974;32:362-364.

  6. Mukoyama M, Lida M, Sobue I. Hyperbaric oxygen therapy for peripheral nerve damage induced in rabbits with clioquinol. Exp Neurol. 1975;47:371-380.

  7. Holbach KH, Wassmann H, Linke D. The use of hyperbaric oxygenation in the treatment of spinal cord lesions. Eur Neurol. 1977;16(1-6):213-21.

  8. Jain, KK. Textbook on Hyperbaric Medicine. 1999;478-480.

  9. Kihara M, McManis PG, Schmelzer JD, Kihara Y, Low PA. Experimental ischaemic neuropathy: salvage with hyperbaric oxygenation. Ann Neurol. 1995;37 (1):89-94.

  10. Zamboni WA, Brown RE, Roth AC, Mathur A, Stephenson LL. Functional evaluation of peripheral nerve repair and the effect of hyperbaric oxygen. J Reconstr Microsurg. 1995;11(1):27-9.

  11. Bradshaw PO, Nelson AG, Fanton JW, Yates T, Kagan-Hallet KS. Effect of hyperbaric oxygenation on peripheral nerve regeneration in adult male rabbits. Undersea Hyperb Med. 1996;23(2):107-13.

  12. Haapaniemi T, Nylander G, Kanje M, Dahlin L. Hyperbaric oxygen treatment enhances regeneration of the rat sciatic nerve. Exp Neurol. 1998;149(2):433-8.

  13. HaapaniemiT, Nishiura Y, Dahlin LB. Effects of hyperbaric oxygen treatment on axonal outgrowth in sciatic nerve grafts in rats. Scand J Plast Reconstr Surg Hand Surg. 2001;35(1):7-11.

  14. Bajrovic FF, Sketelj J, Jug M, Gril I, Mekjavic IB. The effect of hyperbaric oxygen treatment on early regeneration of sensory axons after nerve crushes in the rat. J Peripher Nerv Syst. 2002;7(3):141-48.

  15. Perez-Bolde A, Sanchez EC. Hyperbaric oxygen therapy in the peripheral nerve regeneration. Undersea and Hyperbaric Medicine. 1999;26-40.

  16. Liu, QL, HeB P.Effects of hyperbaric oxygen therapy on rat sciatic nerve injury. Undersea and Hyperbaric Medicine Biomed Res. 1994; 21:3341-343.

  17. Tyshkevic TG, Bersnev VP, Rafikov AM. The prospects for the sequential use of hyperbaric oxygenation and muscle electrostimulation for the rehabilitation of patients with nerve injuries. Vopr Kurortol Fisioter Lech Fiz Kult. 1990;(4):26-30.

  18. Zhao DW. Therapeutic effect of hyperbaric oxygen on recovery of surgically repaired peripheral nerve injury. Zhonghua Wai Ke Za Zhi. 1991;29(2):118-120, l43.

  19. Najajima M, Kuwabara S, Uchino F, Hiryama K. Enhanced regeneration of terminal axons after hyperbaric oxygen therapy in a patient resembling progressive postpoliomyelitis muscular atrophy. Rinsho Shinkeigaku. 1994;34(1):48-51.

  20. Gen J, Wang YW, Gao HJ. Hyperbaric oxygen therapy of nerve injuries: 3 case report. J Xi An Med Coll. 1982;6:797-798.

  21. Viera C, Galvez C, Carrasco B, Santos C, Castellanos R. A study of peripheral neural conduction, motor and sensory in diabetic patients treated with hyperbaric oxygenation. Rev Neurol. 1999;28(9):868-72.

  22. Ochs, S. Energy metabolism and supply of P to the fast axoplasmic transport mechanism in nerve. Fed Proc. 1974;33:1049-1058.

  23. Marx R.E, Ehler WJ, Tayapongsak P, Pierce LW. Relationship of oxygen dose to angiogenesis induction in irradiated tissue. Am J of Surg. 1990;160:519-524.

  24. Knighton DR, Silver IA, Hunt TK. Regulation of wound angiogenesis. Effect of oxygen gradients and inspired oxygen concentration. Surg. 1981;90(2):262-269.

  25. Polverini PJ, Coran RS, Ginbrone MA, Unanue ER. Activated macrophages induced vascular proliferation. Nature. 1977;269:804.

  26. Thakral KK, Goodson WH, Hunt TK. Stimulation of wound blood vessel growth by wound macrophages. J Surg Res. 1979;26:430.

  27. Kim ED, Scardino PT, Hampfel O. Interposition of sural nerve restores function of cavernous nerves resected during radical prostatectomy. J Urol. 1999; 161:188.

  28. Kim ED, Nath R, Kadmon D. Bilateral nerve graft during radical retropubic prostatectomy: 1 year follow-up. J Urol. 2001;165(6pt1):1950-1956.




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