Nachón GFJ, Díaz TJ, Rivas EV, González HJS, Nachón GMG, García GF, Santiago GJ

Language: Spanish
References: 26
Page: 5-10
PDF size: 315.49 Kb.

ABSTRACT

The technological improvements in the operational and diagnostic optic systems, as well as the surgical instruments, are in need of the use of materials such as plastic and optic fi ber in their manufacture. A lot of these materials do not resist pressure steam sterilization, therefore it is needed alternative methods that are to be fast, at a low cost and highly eff ective, characteristics that have been met with the chemical substances immersion sterilization method. It is intended in the current paper to assess and compare the in vitro sterilizing activity of 2% glutaraldehyde (GA 2%), superoxydated electrolized water (AES) and sterilizing electrolized solution by ionic selectivity (SESI), through the elimination of microorganisms of the surgical instruments contaminated by non-attenuated pathogens obtained in hospital environment. It was prepared contaminating solutions with E.coli, P. aeruginosa, Metilicinresistant S. aureus and C. albicans in concentrations of 1 x 10⁸ CFU per cc in sterile saline, into which the surgical instrument was submerged for 15 minutes and afterwards it was exposed to each of the sterilizing solutions at three different times 7.5, 10 and 15 minutes. After the 15 minutes of immersion, all the cultures made to the instruments submitted to SEESI and 2% GA turned out negative differing from the 4 positive cultures of the instruments submitted to AES p‹ 0.05. Due to the latest it can be said that the instruments treated with SESI and 2% GA met the sterilization requirements.

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