medigraphic.com
Órgano Oficial del Instituto de Ciencias de la Salud, Hospital Escuela y Facultad de Medicina-Xalapa

2008, Number 1

<< Back Next >>

Rev Med UV 2008; 8 (1)

The yellow wasp (Polistes mexicana): new vector of actinomycetum in mycetoma

Martínez RA, Cruz LH, Aburto SMA, Isauro GL, Camacho MA, Gómez FM

Language: Spanish
References: 21
Page: 19-24
PDF size: 751.98 Kb.


ABSTRACT

A 47 year-old woman developed a painful wound with fistulae characteristic of mycetoma in the left side of the dorsal region. A skin biopsy of the lesion showed characteristics grains in PAS and Grocott stains, and the Sabouraud agar culture showed growth of colonies of Nocardia. Therefore, treatment with sulfas, amoxicillin and cephalosporins was indicated to the patient. The patient reported to be a bite by a yellow wasp (Polistes mexicana) in the dorsum in the left side at D7 level and refers to have developed a localized fistulae injury in centrifugal form to the wasp bite. The objective of the present study is to demonstrate if the yellow wasp (Polistes mexicana) is a possible vector of actinomycetum in mycetoma.
Then we begin collection of wasps in the site where the patient was assailed; the wasps were incubating in Sabouraud agar at 37 ºC, were detected microbial growth characteristic of actinomycetum and stain of Gram and Ziehl Neelsen detected and showed hyphas and coccibacillus gram positives and resistant alcohol-acid. The strain was purified and hydrolysis casein test it was resulted positive to Nocardia brasiliensis. Thus, the study showed that the yellow wasp (Polistes mexicana) could be the responsive of inoculate actinomycetum with its bite and to cause mycetoma development so; the yellow wasp (Polistes mexicana) is a vector of actinomycetum in mycetoma.


REFERENCES

  1. Vera L, Salinas M, Welsh O. Isolation and Purification of Two Immunodominant Antigensfrom Nocardia brasiliensis. J Clin Microbiol. 1992; 1183-8.

  2. Rodríguez M, Alvarado A, Del Carmen M, Ramos A. Micetoma de inoculación múltiple por Nocardia brasiliensis. Reporte de un caso. Rev Cent Dermatol Pascua. 2002; 11, (3): 126-30.

  3. Salinas M, Torres E, Ramos A. Immune Response toNocardia brasiliensis Antigens in an Experimental Model of Actinomycetoma in BALB/c mice. Infect Immun. 1999; 67 (5): 2428–32.

  4. Lupi O, Tyring S, McGinnis M. Tropical dermatology: Fungal tropical diseases. J Am Acad Dermatol. 2005; 53 (6): 931-51.

  5. Mendez L, Mondragón R, Manzano P. Inmunoglobulinas en pacientes con actinomicetoma por Nocardia brasiliensis, Rev Argent Microbiol. 2004; 36: 174-8.

  6. Arenas R. Dermatología Atlas Diagnóstico y tratamiento. 2ª Ed. Mc Graw Hill Interamericana. 2000. México. 1- 642.

  7. Saúl A. Lecciones de Dermatología. 14 Ed. Méndez Editores. 2000. México. 1 - 783.

  8. Maraki S, Chochlidakis S, Nioti E, Tselentis Y. Primary lymphocutaneous nocardiosis in an immunocompetent patient Ann Clin Microbiol Antimicrob. 2004; 3 (24): 1-4

  9. Salinas M, Castro M, Sepulveda J. Monoclonal antibodies to P24 and P61 immunodominant antigens from Nocardia brasiliensis. ClinDiagnostic Lab Immunol. 1997; 4 (2): 133–7.

  10. Isik K, Chun J, Chil Y, Goodfellow M. Nocardia uniformis nom. rev. Int J Syst Bacteriol. 1999; 49: 227–30.

  11. IşiIk K, Kariptaş E. Identification and evaluation ofclinically significant Nocardia brasiliensis. Turk J Biol. 2001; 25: 103-11.

  12. Palma A, Castrillón L, Padilla C, Reyes F. Caracterización histoquímica de micetomas por Actinomadura madurae, nocardia brasiliensis y Madurella mycetomatis Dermatología Rev Mex. 2005; 49 (2): 51-8.

  13. A. Saubolle M., Sussland D. Nocardiosis: Review of clinical and laboratory experience. J Clin Microbiol. 2003; 41 (10): 4497–501.

  14. Matulionyte R, Rohner P, Uçkay I, Lew D, Garbino J, Secular trends of nocardia infection over 15 years in a tertiary care hospital. J Clin Pathol. 2004; 57: 807-12.

  15. Gómez-Flores A, Welsh O, Said-Fernandez S, et al. In Vitro and In Vivo activities of antimicrobials against Nocardia brasiliensis. Antimicrob Agents and Chemother. 2004; 48 (3): 832-7.

  16. Salinas M, Welsh O, Casillas S. Enzyme-Linked Immunosorbent assay for serological diagnosis of Nocardia brasiliensis and Clinical Correlation with Mycetoma Infections. J Clin Microbiol. 1993; 31 (11): 2901-6.

  17. Salinas M. Anticuerpos anti-Nocardia brasiliensis en pacientes con actinomicetoma y su utilidad clínica, Gac Med Mex. 2001; 137 (1): 1- 8.

  18. Vera L, Johnson W, Welsh O. Distribution of a Nocardia brasiliensis Catalase Gene Fragment in Members of the Genera Nocardia, Gordona, and Rhodococcus. J Clin Microbiol. 1999; 37 (6): 1971–6.

  19. Salinas M, Perz I. Humoral immunity through immunoglobulin M protects mice from an experimental actinomycetoma infection by Nocardia brasiliensis. Infect Immun. 2004; 72 (10): 5597–604.

  20. Conde C, Mancilla R, Ortiz L. Immunoglobulin and complement in tissues of mice infected with Nocardia brasiliensis. Infect Immun 1983; 40 (3): 1218-22.

  21. Conde C, Melendra E, Fresan M, Ortiz L. Nocardia brasiliensis: Mycetoma induction and growth cycle. Infect Immun. 1982; 38 (3): 1291-5.




2020     |     www.medigraphic.com