Chávez BE, García VMR, Avelino FF, Gil JC, Castañeda REI

Language: Spanish
References: 14
Page: 114-117
PDF size: 194.42 Kb.

ABSTRACT

Escherichia coli enteropathogenic (EPEC) is the diarrheagenic pathotype cause of the highest morbidity in children and adults, it counts with Bundle-Forming Pilus (BFP) as a virulence attribute. This pili attaches the bacterium to the epithelial cells and trigger a set of transduction signals causing the colonization and disease. EPEC have other virulence factors, no pathologic by their selves, but essential to the bacteria surviving, like siderophores production and heavy-metal resistance. In this work the bfpA gene, the siderophores production and the heavy-metal resistance, were reviewed in 48 E. coli strains from adult patients from Puebla – México; 30% of the strains amplified bfpA gene, 89,6% were positive to siderophores production and all of them offered resistance to heavy metals (Fe, Co, Mo, Pb, Ni, Cu, Hg and V); 25% presented three virulence factors, 63% single siderophores production and resistance to heavy metals. Our results agree with that reported by Kuhnert et al. (1999), who propose that virulence genes help EPEC to adapt to host and express itself during infection process, being facilitated adhesion, invasion or both. We demonstrated in this study too, that EPEC expressed at least two virulence factors in E. coli strains isolated from adult patients form Puebla – Mexico.

REFERENCES

1. Gyles C.L. “Escherichia coli in domestic animals and humans.” CAB International, 1994; Wallingford, UK.

2. Johnson J.R. “Virulence factors in Escherichia coli urinary tract infection.” Clinical Microbiology Reviews, 1991; 4:80-128.

3. Donnenberg MS and Kaper JP.: “Enteropathogenic Escherichia coli.” Infect. Immun., 1992; 60(10): 3953-3961.

4. Guerrant RL, Hugues JM, Lima NL, and Crane J. “Diarrhea in developed and developing countries: Magnitude, special settings, and etiologies.” Rev Infect Dis., 1990; 12(1): S41-S50.

5. Kaper J.B. and O´Brien A.D. Escherichia coli O157:H7 and other shiga toxin-producing E. coli strains. 1998; ASM Press, Washington, USA.

6. Puente J. L., D. Bieker, S. W. Ramer, W. Murray and G. K. Schoolnik. “The bundle forming pili of enteropathogenic Escherichia coli: transcriptional regulation by environmental signals.” Molec. Microb. 1996; 20: 87-100

7. Faraldo Gómez J.D. and Sansom Mark S.P. “Adquisition of siderophores in gram-negative bacteria.” Rev. Nature, Molecular cell Biology. 2003; 4: 105-116.

8. Kasan H. “The role of wastw activated sludge and bacteria in metal-ion renoval from solution.” Crit. Rev. Environ. Sci. Technol, 1993; 23:79-117.

9. Jackson T, West M, Leppard G. “Accumulationof heavy metals by individually analyzed bacterial cells and associated non- living material in polluted lake sediments.” Environ. Sci. Technol, 2000; 33: 3795-3801.

10. Silver S. “Exploting heavy metal resistance systems in bioremediation.” Res.Microbiol. 1994; 195: 61-66.

11. Filali BK, Taoufik J, Zeroual Y, Dzairi FZ, Talbi M, Blaghen M. “Waste water bacterial isolates resistant to heavy metals and antibiotics.” Cur Microbiol 2000; 41: 151-156.

12. Valdespino Gómez J. l. García García M. de l; Del Río Zolezzi A; Giono Cerezo S; Salcedo Alvarez R. A; Sepúlveda Amor J; “Epidemiología y etiología de las diarreas Infecciosas. El caso de México.” Rev Latinoam Microbial, 1994; 36:307-324.

13. Nataro J.P. and Kaper J.B. “Diarrheagenic Escherichia coli. Clinical.” Microbiology Reviews, 1998; 11:142-201.

14. Casadevall A. and Pirofski L. “Host pathogen interactions: basic concepts of microbial comensalism, colonization, infection and disease.” Infect immune, 2000; 68:6511-6518.